PMID- 23828471
OWN - NLM
STAT- MEDLINE
DCOM- 20140409
LR  - 20190923
IS  - 1678-2925 (Electronic)
IS  - 0004-2749 (Linking)
VI  - 76
IP  - 2
DP  - 2013 Mar-Apr
TI  - Light-induced retinal injury enhanced neurotrophins secretion and neurotrophic 
      effect of mesenchymal stem cells in vitro.
PG  - 105-10
LID - S0004-27492013000200010 [pii]
AB  - PURPOSE: To investigate neurotrophins expression and neurotrophic effect change 
      in mesenchymal stem cells (MSCs) under different types of stimulation. METHODS: 
      Rats were exposed in 10,000 lux white light to develop light-induced retinal 
      injury. Supernatants of homogenized retina (SHR), either from normal or 
      light-injured retina, were used to stimulate MSCs. Quantitative real time for 
      polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) 
      were conducted for analysis the expression change in basic fibroblast growth 
      factor (bFGF), brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic 
      factor (CNTF) in MSCs after stimulation. Conditioned medium from SHR-stimulated 
      MSCs and control MSCs were collected for evaluation their effect on retinal 
      explants. RESULTS: Supernatants of homogenized retina from light-injured rats 
      significantly promoted neurotrophins secretion from MSCs (p<0.01). Conditioned 
      medium from mesenchymal stem cells stimulated by light-injured SHR significantly 
      reduced DNA fragmentation (p<0.01), up-regulated bcl-2 (p<0.01) and 
      down-regulated bax (p<0.01) in retinal explants, displaying enhanced protective 
      effect. CONCLUSIONS: Light-induced retinal injury is able to enhance 
      neurotrophins secretion from mesenchymal stem cells and promote the neurotrophic 
      effect of mesenchymal stem cells.
FAU - Xu, Wei
AU  - Xu W
AD  - Department of Ophthalmology, First Affiliated Hospital of Fujian Medical 
      University, Fuzhou City, China.
FAU - Wang, Xiaoting
AU  - Wang X
FAU - Xu, Guoxing
AU  - Xu G
FAU - Guo, Jian
AU  - Guo J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Arq Bras Oftalmol
JT  - Arquivos brasileiros de oftalmologia
JID - 0400645
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Ciliary Neurotrophic Factor/metabolism
MH  - Disease Models, Animal
MH  - Fibroblast Growth Factors/metabolism
MH  - *Light
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Retina/cytology/injuries/*radiation effects
EDAT- 2013/07/06 06:00
MHDA- 2014/04/10 06:00
CRDT- 2013/07/06 06:00
PHST- 2012/09/24 00:00 [received]
PHST- 2013/01/31 00:00 [accepted]
PHST- 2013/07/06 06:00 [entrez]
PHST- 2013/07/06 06:00 [pubmed]
PHST- 2014/04/10 06:00 [medline]
AID - S0004-27492013000200010 [pii]
AID - 10.1590/s0004-27492013000200010 [doi]
PST - ppublish
SO  - Arq Bras Oftalmol. 2013 Mar-Apr;76(2):105-10. doi: 
      10.1590/s0004-27492013000200010.