PMID- 23829296
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20130916
IS  - 1399-0004 (Electronic)
IS  - 0009-9163 (Linking)
VI  - 84
IP  - 4
DP  - 2013 Oct
TI  - Molecular cytogenetics: recent developments and applications in cancer.
PG  - 315-25
LID - 10.1111/cge.12229 [doi]
AB  - Aneuploidy or alteration in chromosome numbers is a characteristic feature in 
      cancer that is generally a consequence of defective chromosome segregation during 
      cell division. Molecular cytogenetic analyses have conferred substantial evidence 
      with regards to the chromosomal architectures in cancer. Most importantly, the 
      fluorescence in situ hybridization (FISH) technique that plays a leading role in 
      diagnostic pathology for its single-cell analysis has provided crucial 
      information regarding genomic variations in malignant cells. Further development 
      of molecular cytogenetic methodologies such as chromosome specific FISH 
      karyotyping and comparative genomic hybridization have also helped in the 
      detection of cryptic genetic changes in cancer. But, the recent advancement of 
      high throughput sequencing technologies have provided a more comprehensive 
      genomic analyses resulting in novel chromosome rearrangements, somatic mutations 
      as well as identification of fusion genes leading to new therapeutic targets. 
      This review highlights the application of early molecular cytogenetics and the 
      recent high throughput genomic approaches in characterizing various cancers and 
      their invaluable support in cancer therapeutics.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Das, K
AU  - Das K
AD  - Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore, 
      Singapore.
FAU - Tan, P
AU  - Tan P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20130806
PL  - Denmark
TA  - Clin Genet
JT  - Clinical genetics
JID - 0253664
SB  - IM
MH  - *Cytogenetics/methods
MH  - Humans
MH  - *Molecular Diagnostic Techniques
MH  - Neoplasms/*diagnosis/*genetics
OTO - NOTNLM
OT  - CGH
OT  - M-FISH
OT  - array-CGH
OT  - cancer
OT  - fluorescence in situ hybridization
OT  - genomics
OT  - molecular cytogenetics
OT  - next generation sequencing
OT  - spectral karyotyping
EDAT- 2013/07/09 06:00
MHDA- 2014/04/09 06:00
CRDT- 2013/07/09 06:00
PHST- 2013/04/23 00:00 [received]
PHST- 2013/07/02 00:00 [revised]
PHST- 2013/07/02 00:00 [accepted]
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
AID - 10.1111/cge.12229 [doi]
PST - ppublish
SO  - Clin Genet. 2013 Oct;84(4):315-25. doi: 10.1111/cge.12229. Epub 2013 Aug 6.