PMID- 23830393
OWN - NLM
STAT- MEDLINE
DCOM- 20130904
LR  - 20130708
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 27
IP  - 2
DP  - 2013 Apr-Jun
TI  - Glucocorticoids upregulate decreased IL-7 receptor expression in asthmatic 
      patients and simian immunodeficiency virus-infected non-human primates.
PG  - 427-42
AB  - Signaling through interleukin-7 receptor (IL-7R) is essential for regulation of 
      T-cell homeostasis and survival. Previously, we and others have found diminished 
      IL-7R levels in simian immunodeficiency virus (SIV) - infected non-human primates 
      and human immunodeficiency virus (HIV) - infected patients. To date, it remains 
      unknown whether changes in IL-7R expression could also be linked to 
      non-infectious inflammatory diseases such as asthma or anti-inflammatory drug 
      use. Here, we investigated through flow cytometry the levels of IL-7R expression 
      on CD4+ and CD4- T-cells in asthmatic patients in relation to disease severity, 
      immune status and glucocorticoid (GC) use. In addition, we sought to evaluate the 
      effects of in vivo and in vitro GC treatment on IL-7R expression in both 
      asthmatic patients and SIV-infected non-human primates. We demonstrated that 
      expression of IL-7R on peripheral blood CD4+ T-cells was significantly decreased 
      in clinically stable GC-naive mild and moderate asthmatic patients. Accordingly, 
      the development of asthmatic reaction following bronchial allergen challenge 
      performed in sensitized subjects was associated with a significant drop in levels 
      of IL-7R on circulating CD4+ T-cells. In contrast, CD4+ T-cells from both, mild 
      and moderate, but not severe asthmatics, treated with inhaled GC displayed levels 
      of IL-7R similar to that seen in healthy controls. We did not find significant 
      differences with serum or sputum interleukin-7 levels among healthy controls and 
      GC-naive and GC-treated asthmatic patients. Furthermore, both in vitro GC 
      treatment and short-term oral GC administration to asthmatic patients resulted in 
      a significant enhancement of IL-7R. Similarly, we demonstrated that GC-stimulated 
      T-cells from SIV-infected non-human primates up-regulated IL-7R expression. 
      Accordingly, experimental short-term systemic in vivo administration of GC to 
      SIV-infected macaques led to enhancement of IL-7R expression on circulating 
      T-cells. Our data indicate that GC bear potential to recover diminished IL-7R 
      expression, as well in asthma as in lentiviral infection.
FAU - Moniuszko, M
AU  - Moniuszko M
AD  - Department of Allergology and Internal Medicine, Medical University of Bialystok, 
      Bialystok, Poland. moniuszm@umb.edu.pl
FAU - Lipinska, D
AU  - Lipinska D
FAU - Jeznach, M
AU  - Jeznach M
FAU - Kowal, K
AU  - Kowal K
FAU - Grubczak, K
AU  - Grubczak K
FAU - Rusak, M
AU  - Rusak M
FAU - McKinnon, K
AU  - McKinnon K
FAU - Vaccari, M
AU  - Vaccari M
FAU - Liyanage, N P M
AU  - Liyanage NP
FAU - Fenizia, C
AU  - Fenizia C
FAU - Wawrusiewicz-Kurylonek, N
AU  - Wawrusiewicz-Kurylonek N
FAU - Dabrowska, M
AU  - Dabrowska M
FAU - Jablonska, E
AU  - Jablonska E
FAU - Kretowski, A
AU  - Kretowski A
FAU - Gorska, M
AU  - Gorska M
FAU - Bodzenta-Lukaszyk, A
AU  - Bodzenta-Lukaszyk A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Glucocorticoids)
RN  - 0 (Interleukin-7)
RN  - 0 (Receptors, Interleukin-7)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Asthma/drug therapy/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Glucocorticoids/*pharmacology
MH  - Humans
MH  - Interleukin-7/blood
MH  - Macaca mulatta
MH  - Middle Aged
MH  - Receptors, Interleukin-7/*analysis
MH  - Simian Acquired Immunodeficiency Syndrome/*immunology
EDAT- 2013/07/09 06:00
MHDA- 2013/09/05 06:00
CRDT- 2013/07/09 06:00
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2013/09/05 06:00 [medline]
AID - 15 [pii]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2013 Apr-Jun;27(2):427-42.