PMID- 23830396
OWN - NLM
STAT- MEDLINE
DCOM- 20130904
LR  - 20151119
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 27
IP  - 2
DP  - 2013 Apr-Jun
TI  - T-cell subpopulations express a different pattern of dopaminergic markers in 
      intra- and extra-thymic compartments.
PG  - 463-75
AB  - An involvement of dopamine in regulation of the immune function has been assessed 
      and dopaminergic system has been found widely represented in thymus. Nevertheless 
      detail on the characterization of dopaminergic system in assisting thymocytes 
      development and lymphocytes mature physiology are still lacking. The present 
      study was designed to characterize dopamine plasma membrane transporter (DAT), 
      vesicular dopamine transporters (VMAT)-1 and -2, and dopamine D1-like and D2-like 
      receptors in rat thymocytes, splenocytes and peripheral blood mononuclear cells. 
      Western blot and RT-PCR analyses, performed on these cells, showed an expression 
      of dopamine transporters and receptors during thymocyte development (when of CD4 
      and CD8 markers are differently expressed). Furthermore FACS analysis, indicates 
      that DAT and dopamine D1-like receptors are expressed at high levels in 
      thymocytes, splenocytes, and peripheral lymphocytes. The percentage of CD4+ CD8+ 
      (double-positive) thymocytes expressing dopaminergic markers was significantly 
      higher compared to the percentage of double-negative ones. The percentage of CD8+ 
      single positive cells expressing dopaminergic markers was significantly higher 
      than that of CD4+ cells. The results suggest that the dopaminergic system plays a 
      role in the thymus microenvironment during T-cell development. The more 
      pronounced expression of dopaminergic markers in CD8+ subsets suggests that 
      dopamine plays a role in development of cytotoxic T-cells. Our findings indicate 
      dopaminergic system to have a role during the maturation and selection of 
      lymphocytes, and support its involvement in the active phases of immune response.
FAU - Mignini, F
AU  - Mignini F
AD  - School of Pharmacy, University of Camerino, Camerino, Italy. 
      fiorenzo.mignini@unicam.it
FAU - Sabbatini, M
AU  - Sabbatini M
FAU - Capacchietti, M
AU  - Capacchietti M
FAU - Amantini, C
AU  - Amantini C
FAU - Bianchi, E
AU  - Bianchi E
FAU - Artico, M
AU  - Artico M
FAU - Tammaro, A
AU  - Tammaro A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Biomarkers)
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Receptors, Dopamine D1)
RN  - 0 (Receptors, Dopamine D2)
RN  - 0 (Slc18a1 protein, rat)
RN  - 0 (Slc18a2 protein, rat)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Dopamine/*physiology
MH  - Dopamine Plasma Membrane Transport Proteins/analysis
MH  - Flow Cytometry
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Dopamine D1/analysis
MH  - Receptors, Dopamine D2/analysis
MH  - T-Lymphocyte Subsets/*chemistry/immunology
MH  - Vesicular Monoamine Transport Proteins/analysis
EDAT- 2013/07/09 06:00
MHDA- 2013/09/05 06:00
CRDT- 2013/07/09 06:00
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2013/09/05 06:00 [medline]
AID - 18 [pii]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2013 Apr-Jun;27(2):463-75.