PMID- 23830992
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20140203
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 116
IP  - 1
DP  - 2014 Jan
TI  - Reduced expression of Munc13-1 in human and porcine diabetic peripheral nerve.
PG  - 106-11
LID - S0065-1281(13)00109-8 [pii]
LID - 10.1016/j.acthis.2013.05.010 [doi]
AB  - Peripheral neuropathy (PN) involves widespread peripheral nerve disorders 
      affecting a large human population worldwide. In Europe and the United States, 
      the first single most prominent cause of peripheral neuropathy is diabetes, 
      affecting 60-70% patients with long-term diabetes followed by idiopathic 
      neuropathy, peripheral nerve damage of unknown etiology, diagnosed in 10-40% of 
      all patients admitted to hospitals with symptoms of peripheral nerve damage. The 
      molecular mechanisms underlying the pathogenesis of this disorder are not yet 
      fully understood, however a few potential molecular contributors, such as 
      Munc13-1, have been recently suggested. Munc13-1 is a diacylglycerol (DAG) 
      receptor and a multifunction active zone protein essential for synaptic vesicle 
      priming and crucial for insulin release from pancreatic beta cells. Here, for the 
      first time, we focused on the comparative expression of Munc13-1 in human and 
      porcine peripheral nerves. Our results revealed significantly reduced number of 
      Munc13-1 in human (64.26% +/- 6.68%) and porcine (84.09% +/- 2.21%) diabetic nerve 
      fibers and lower number of the double stained, neuronal marker, Neurofilament 
      (NF) and Munc13-1 positive, human (56.83% +/- 3.77%) and porcine (65.87% +/- 4.86%) 
      nerve fibers. Optical density quantification of Western blots showed similar 
      results. Our study indicates that Munc13-1, on account of its role in both 
      insulin and neurotransmitter exocytosis and through its binding properties, may 
      be an important factor contributing to the development or progression of diabetic 
      neuropathy.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - Juranek, Judyta K
AU  - Juranek JK
AD  - Department of Surgery, Columbia University Medical Center, New York, USA; 
      Diabetes Research Program, Department of Medicine, NYU Medical Center, New York, 
      USA. Electronic address: judytajuranek@gmail.com.
FAU - Geddis, Matthew S
AU  - Geddis MS
AD  - Department of Surgery, Columbia University Medical Center, New York, USA; 
      Department of Science, BMCC-City University of New York, New York, USA.
FAU - Kothary, Pratik
AU  - Kothary P
AD  - Department of Surgery, Columbia University Medical Center, New York, USA; 
      Diabetes Research Program, Department of Medicine, NYU Medical Center, New York, 
      USA.
FAU - Hays, Arthur
AU  - Hays A
AD  - Department of Pathology, Columbia University Medical Center, New York, USA.
FAU - Schmidt, Ann Marie
AU  - Schmidt AM
AD  - Department of Surgery, Columbia University Medical Center, New York, USA; 
      Diabetes Research Program, Department of Medicine, NYU Medical Center, New York, 
      USA.
FAU - Brannagan, Thomas H 3rd
AU  - Brannagan TH 3rd
AD  - Department of Neurology, Columbia University Medical Center, New York, USA.
LA  - eng
PT  - Journal Article
DEP - 20130705
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (UNC13B protein, human)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/complications/*metabolism
MH  - Diabetic Neuropathies/*metabolism
MH  - Humans
MH  - Intermediate Filaments/metabolism
MH  - Male
MH  - Middle Aged
MH  - Nerve Tissue Proteins/*metabolism
MH  - Peripheral Nerves/metabolism
MH  - Sus scrofa
OTO - NOTNLM
OT  - Diabetes
OT  - Human
OT  - Immunofluorescence
OT  - Munc13-1
OT  - Peripheral neuropathy
OT  - Pig
EDAT- 2013/07/09 06:00
MHDA- 2015/05/15 06:00
CRDT- 2013/07/09 06:00
PHST- 2013/03/03 00:00 [received]
PHST- 2013/05/28 00:00 [revised]
PHST- 2013/05/29 00:00 [accepted]
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
AID - S0065-1281(13)00109-8 [pii]
AID - 10.1016/j.acthis.2013.05.010 [doi]
PST - ppublish
SO  - Acta Histochem. 2014 Jan;116(1):106-11. doi: 10.1016/j.acthis.2013.05.010. Epub 
      2013 Jul 5.