PMID- 23831194
OWN - NLM
STAT- MEDLINE
DCOM- 20140328
LR  - 20130819
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 59
DP  - 2013 Sep
TI  - Free radical scavenging and anti-oxidative activities of an ethanol-soluble 
      pigment extract prepared from fermented Zijuan Pu-erh tea.
PG  - 527-33
LID - S0278-6915(13)00426-2 [pii]
LID - 10.1016/j.fct.2013.06.047 [doi]
AB  - An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh 
      tea. The compositions of the ethanol soluble pigment extract were analyzed by 
      high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS). The 
      extract was prepared into a series of ethanol solutions and analyzed for free 
      radical-scavenging activities (against two free radicals: 
      1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl 
      (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance 
      spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing 
      extract concentration, suggesting that the extract had excellent free 
      radical-scavenging activities. In vitro cell culture suggested that, at 50-200 
      mg/L, the extract had no measurable effect on the viability of vascular 
      endothelial cells (ECV340) but produced significant protective effects for cells 
      that underwent oxidative injuries due to hydrogen peroxide (H(2)O(2)) treatment. 
      Compared with the H(2)O(2) treatment alone cells group, 200 mg/L of the extract 
      increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and 
      decreased the concentration of malondialdehyde (MDA) and the activity of lactate 
      acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest 
      that the extract has excellent free radical scavenging and anti-oxidative 
      properties.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Fan, Jiang Ping
AU  - Fan JP
AD  - College of Food Science and Technology, Yunnan Agricultural University, Kunming 
      650201, China.
FAU - Fan, Chong
AU  - Fan C
FAU - Dong, Wen Min
AU  - Dong WM
FAU - Gao, Bin
AU  - Gao B
FAU - Yuan, Wei
AU  - Yuan W
FAU - Gong, Jia Shun
AU  - Gong JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130703
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Antioxidants)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Pigments, Biological)
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 0 (Tea)
RN  - 3K9958V90M (Ethanol)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.1.- (Lactate Dehydrogenases)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Antioxidants/chemistry/isolation & purification/metabolism/*pharmacology
MH  - Camellia sinensis/chemistry/metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - China
MH  - Diet/ethnology
MH  - Endothelium, Vascular/cytology/*drug effects/enzymology/metabolism
MH  - Ethanol/chemistry
MH  - Ethnopharmacology
MH  - Fermentation
MH  - Food Handling
MH  - Free Radical Scavengers/adverse effects/chemistry/isolation & 
      purification/pharmacology
MH  - Humans
MH  - Lactate Dehydrogenases/chemistry/*metabolism
MH  - Malondialdehyde/antagonists & inhibitors/metabolism
MH  - Oxidative Stress/drug effects
MH  - Pigments, Biological/adverse effects/chemistry/isolation & 
      purification/*pharmacology
MH  - Plant Extracts/adverse effects/chemistry/isolation & purification/*pharmacology
MH  - Solubility
MH  - Solvents/chemistry
MH  - Superoxide Dismutase/chemistry/*metabolism
MH  - Tea/*chemistry/microbiology
OTO - NOTNLM
OT  - Anti-oxidation
OT  - Ethanol-soluble pigment
OT  - Free radical
OT  - Zijuan Pu-erh tea
EDAT- 2013/07/09 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/07/09 06:00
PHST- 2013/02/15 00:00 [received]
PHST- 2013/06/19 00:00 [revised]
PHST- 2013/06/23 00:00 [accepted]
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - S0278-6915(13)00426-2 [pii]
AID - 10.1016/j.fct.2013.06.047 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2013 Sep;59:527-33. doi: 10.1016/j.fct.2013.06.047. Epub 2013 
      Jul 3.