PMID- 23832111
OWN - NLM
STAT- MEDLINE
DCOM- 20141022
LR  - 20220311
IS  - 1590-3478 (Electronic)
IS  - 1590-1874 (Linking)
VI  - 35
IP  - 3
DP  - 2014 Mar
TI  - Bone marrow stromal cells promote neurite outgrowth of spinal motor neurons by 
      means of neurotrophic factors in vitro.
PG  - 449-57
LID - 10.1007/s10072-013-1490-x [doi]
AB  - Transplantation of bone marrow stromal cells (BMSCs) into spinal cord injury 
      models has shown significant neural function recovery; however, the underlying 
      mechanisms have not been fully understood. In the present study we examined the 
      effect of BMSCs on neurite outgrowth of spinal motor neuron using an in vitro 
      co-culture system. The ventral horn of the spinal grey matter was harvested from 
      neonatal Sprague-Dawley rats, cultured with BMSCs, and immunostained for 
      neurofilament-200 (NF-200). Neurite outgrowth of spinal motor neurons was 
      measured using Image J software. ELISA was used to quantify neurotrophic factors 
      such as brain-derived neurotrophic factor (BDNF), glial cell line-derived 
      neurotrophic factor (GDNF) and nerve growth factor (NGF) in culture media, and 
      antibodies or exogenous neurotrophic factors were used to block or mimic the 
      effect of BMSCs on neurite outgrowth, respectively. The results showed that 
      neurite outgrowth significantly increased in spinal motor neurons after 
      co-cultured with BMSCs, while the secretion level of BDNF, GDNF and NGF was 
      dramatically elevated in co-culture. However, the neurite outgrowth-promoting 
      effect of BMSCs was found to significantly reduced using antibodies to BDNF, GDNF 
      and NGF. In addition, a fraction of BMSCs was found to exhibit NF-200 
      immunoreactivity. These results indicated that BMSCs could promote neurite 
      outgrowth of motor neurons by means of neurotrophic factors. The findings of the 
      present study provided new cues for the treatment of spinal cord injury.
FAU - Lin, Weiwei
AU  - Lin W
AD  - Department of Histology and Embryology, Medical College, Nantong University, 19 
      Qixiu Road, Nantong, 226001, JS, People's Republic of China.
FAU - Li, Ming
AU  - Li M
FAU - Li, Yi
AU  - Li Y
FAU - Sun, Xiaoli
AU  - Sun X
FAU - Li, Xiang
AU  - Li X
FAU - Yang, Fan
AU  - Yang F
FAU - Huang, Yuejiao
AU  - Huang Y
FAU - Wang, Xiaodong
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130706
PL  - Italy
TA  - Neurol Sci
JT  - Neurological sciences : official journal of the Italian Neurological Society and 
      of the Italian Society of Clinical Neurophysiology
JID - 100959175
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Neurofilament Proteins)
RN  - 108688-71-7 (neurofilament protein H)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Culture Media, Conditioned/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Mesenchymal Stem Cells/chemistry/*physiology
MH  - Motor Neurons/*cytology/drug effects
MH  - Neurites/drug effects/*physiology
MH  - Neurofilament Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*cytology
EDAT- 2013/07/09 06:00
MHDA- 2014/10/23 06:00
CRDT- 2013/07/09 06:00
PHST- 2012/10/06 00:00 [received]
PHST- 2013/06/24 00:00 [accepted]
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2014/10/23 06:00 [medline]
AID - 10.1007/s10072-013-1490-x [doi]
PST - ppublish
SO  - Neurol Sci. 2014 Mar;35(3):449-57. doi: 10.1007/s10072-013-1490-x. Epub 2013 Jul 
      6.