PMID- 23832878
OWN - NLM
STAT- MEDLINE
DCOM- 20131119
LR  - 20211021
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 61
IP  - 10
DP  - 2013 Oct
TI  - Quantitative analysis of centromeric FISH spots during the cell cycle by image 
      cytometry.
PG  - 699-705
LID - 10.1369/0022155413498754 [doi]
AB  - Two-color fluorescence in situ hybridization (FISH) with chromosome enumeration 
      DNA probes specific to chromosomes 7, 11, 17, and 18 was applied to CAL-51 breast 
      cancer cells to examine whether the fluorescence intensity of FISH spots was 
      associated with cell cycle progression. The fluorescence intensity of each FISH 
      spot was quantitatively analyzed based on the cell cycle stage determined by 
      image cytometry at the single-cell level. The spot intensity of cells in the G2 
      phase was larger than that in the G0/1 phase. This increased intensity was not 
      seen during the early and mid S phases, whereas the cells in the late S phase 
      showed significant increases in spot intensity, reaching the same level as that 
      observed in the G2 phase, indicating that alpha satellite DNA in the centromeric 
      region was replicated in the late S phase. Thus, image cytometry can successfully 
      detect small differences in the fluorescence intensities of centromeric spots of 
      homologous chromosomes. This combinational image analysis of FISH spots and the 
      cell cycle with cell image cytometry provides insights into new aspects of the 
      cell cycle. This is the first report demonstrating that image cytometry can be 
      used to analyze the fluorescence intensity of FISH signals during the cell cycle.
FAU - Amakawa, Genta
AU  - Amakawa G
AD  - Department of Pathology, Yamaguchi University Graduate School of Medicine, Ube, 
      Japan (GA, KI, HI, TF, KS).
FAU - Ikemoto, Kenzo
AU  - Ikemoto K
FAU - Ito, Hideaki
AU  - Ito H
FAU - Furuya, Tomoko
AU  - Furuya T
FAU - Sasaki, Kohsuke
AU  - Sasaki K
LA  - eng
PT  - Journal Article
DEP - 20130704
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
SB  - IM
MH  - *Cell Cycle
MH  - Cell Line, Tumor
MH  - Centromere/*metabolism
MH  - Humans
MH  - *Image Cytometry
MH  - *In Situ Hybridization, Fluorescence
MH  - Spectrometry, Fluorescence
PMC - PMC3788625
OTO - NOTNLM
OT  - DNA ploidy
OT  - FISH
OT  - S phase
OT  - cell cycle
OT  - image cytometry
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2013/07/09 06:00
MHDA- 2013/11/20 06:00
PMCR- 2014/10/01
CRDT- 2013/07/09 06:00
PHST- 2013/07/09 06:00 [entrez]
PHST- 2013/07/09 06:00 [pubmed]
PHST- 2013/11/20 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - 0022155413498754 [pii]
AID - 10.1369_0022155413498754 [pii]
AID - 10.1369/0022155413498754 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2013 Oct;61(10):699-705. doi: 10.1369/0022155413498754. 
      Epub 2013 Jul 4.