PMID- 23834562
OWN - NLM
STAT- MEDLINE
DCOM- 20131030
LR  - 20220309
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 14
DP  - 2013 Jul 9
TI  - A high-resolution cucumber cytogenetic map integrated with the genome assembly.
PG  - 461
LID - 10.1186/1471-2164-14-461 [doi]
AB  - BACKGROUND: High-resolution cytogenetic map can provide not only important 
      biological information on genome organization but also solid foundation for 
      genetic and genomic research. The progress in the molecular and cytogenetic 
      studies has created the basis for developing the cytogenetic map in cucumber 
      (Cucumis sativus L.). RESULTS: Here, the cytogenetic maps of four cucumber 
      chromosomes (chromosomes 1, 3-5) were constructed by fluorescence in situ 
      hybridization (FISH) analysis on cucumber pachytene chromosomes. Together with 
      our previously constructed cytogenetic maps of three cucumber chromosomes 
      (chromosomes 2, 6-7), cucumber has a complete cytogenetic map with 76 anchoring 
      points between the genetic, the cytogenetic and the draft genome assembly maps. 
      To compare our pachytene FISH map directly to the genetic linkage and draft 
      genome assembly maps, we used a standardized map unit-relative map position (RMP) 
      to produce the comparative map alignments. The alignments allowed a global view 
      of the relationship of genetic and physical distances along each cucumber 
      chromosome, and accuracy and coverage of the draft genome assembly map. 
      CONCLUSIONS: We demonstrated a good correlation between positions of the markers 
      in the linkage and physical maps, and essentially complete coverage of chromosome 
      arms by the draft genome assembly. Our study not only provides essential 
      information for the improvement of sequence assembly but also offers molecular 
      tools for cucumber genomics research, comparative genomics and evolutionary 
      study.
FAU - Sun, Jianying
AU  - Sun J
AD  - Institute of Integrative Plant Biology, School of Life Science, Jiangsu Normal 
      University, Xuzhou 221116, China.
FAU - Zhang, Zhonghua
AU  - Zhang Z
FAU - Zong, Xu
AU  - Zong X
FAU - Huang, Sanwen
AU  - Huang S
FAU - Li, Zongyun
AU  - Li Z
FAU - Han, Yonghua
AU  - Han Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130709
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - Cucumis sativus/*genetics
MH  - *Cytogenetic Analysis
MH  - Genetic Linkage/genetics
MH  - Genome, Plant/genetics
MH  - *Genomics
PMC - PMC3710503
EDAT- 2013/07/10 06:00
MHDA- 2013/10/31 06:00
PMCR- 2013/07/09
CRDT- 2013/07/10 06:00
PHST- 2013/05/16 00:00 [received]
PHST- 2013/07/05 00:00 [accepted]
PHST- 2013/07/10 06:00 [entrez]
PHST- 2013/07/10 06:00 [pubmed]
PHST- 2013/10/31 06:00 [medline]
PHST- 2013/07/09 00:00 [pmc-release]
AID - 1471-2164-14-461 [pii]
AID - 10.1186/1471-2164-14-461 [doi]
PST - epublish
SO  - BMC Genomics. 2013 Jul 9;14:461. doi: 10.1186/1471-2164-14-461.