PMID- 23834966
OWN - NLM
STAT- MEDLINE
DCOM- 20140620
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 166
IP  - 1-2
DP  - 2013 Sep 27
TI  - swIFN-beta promotes genetic mutation of porcine reproductive and respiratory 
      syndrome virus in Marc-145.
PG  - 174-8
LID - S0378-1135(13)00312-X [pii]
LID - 10.1016/j.vetmic.2013.06.002 [doi]
AB  - This result, for the first time, demonstrates that under swine interferon-beta 
      (swIFN-beta) immune pressure molecular variation of porcine reproductive and 
      respiratory syndrome virus (PRRSV) accelerates. PRRSV-A1 strain was continuously 
      propagated in Marc-145 cells primed with swIFN-beta for 20 passages to develop 
      RRRSV-A1betaf20 strains while PRRSV-A1f20 was control without swIFN-beta respectively. 
      NSP2, ORF3, ORF5 and ORF6 genes of these strains were amplified, cloned and 
      sequenced. Sequencing analysis indicated that PRRSV-A1betaf20 had 43 nucleotide 
      mutations while PRRSV-A1f20 had only 14 in NSP2, ORF3, ORF5 and ORF6. The ratio 
      of mutative nucleotides between them was 307%. Amino acid variations were 24 in 
      RRRSV-A1betaf20 and 5 in PRRSV-A1, so that mutation in RRRSV-A1betaf20 appeared to be 
      4.8 times faster than that in PRRSV-A1. The ratio of non-synonymous (NS) 
      mutations to synonymous (S) mutations, named NS/S, was 3 in RRRSV-A1betaf20 and 
      0.625 in PRRSV-A1f20. The nucleotide mutation rate of NSP2 ORF3 ORF5 and ORF6 of 
      PRRSV-A1betaf20 were 3.8%, 2.3%, 4.5%, 0.57% and their NS/S were 5, 2, 6 and 1 
      respectively, which is much higher than those in PRRSV-A1f20. Thus the mutative 
      frequency of PRRSV passaged under swIFN-beta immune pressure was significantly 
      faster than that without swIFN-beta. The immuno-pressure of swIFN-beta accelerated 
      genetic variation on PRRSV ORF5, NSP2 and ORF3, but not on the ORF6 gene. PRRSV 
      NSP2 and ORF5 genes may play a key role in escaping from inhibition of swIFN-beta.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - He, Dongsheng
AU  - He D
AD  - College of Veterinary Medicine, South China Agricultural University, Guangdong, 
      Guangzhou 510642, China. dhe@scau.edu.cn
FAU - Niu, Xiaoyun
AU  - Niu X
FAU - Jiang, Aixiang
AU  - Jiang A
FAU - Su, Danping
AU  - Su D
FAU - Zhong, Wang
AU  - Zhong W
FAU - Hu, Jing
AU  - Hu J
FAU - Wang, Yanli
AU  - Wang Y
FAU - Chen, Ruiai
AU  - Chen R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130618
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Viral Proteins)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Genetic Variation
MH  - Interferon-beta/*pharmacology
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/metabolism
MH  - Swine
MH  - Viral Proteins/*genetics/metabolism
OTO - NOTNLM
OT  - Immune pressure
OT  - Mutation
OT  - PRRSV
OT  - swIFN-beta
EDAT- 2013/07/10 06:00
MHDA- 2014/06/21 06:00
CRDT- 2013/07/10 06:00
PHST- 2012/12/31 00:00 [received]
PHST- 2013/05/28 00:00 [revised]
PHST- 2013/06/10 00:00 [accepted]
PHST- 2013/07/10 06:00 [entrez]
PHST- 2013/07/10 06:00 [pubmed]
PHST- 2014/06/21 06:00 [medline]
AID - S0378-1135(13)00312-X [pii]
AID - 10.1016/j.vetmic.2013.06.002 [doi]
PST - ppublish
SO  - Vet Microbiol. 2013 Sep 27;166(1-2):174-8. doi: 10.1016/j.vetmic.2013.06.002. 
      Epub 2013 Jun 18.