PMID- 23835031
OWN - NLM
STAT- MEDLINE
DCOM- 20140418
LR  - 20130916
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 193
IP  - 2
DP  - 2013 Nov
TI  - PK-15 cells transfected with porcine CD163 by PiggyBac transposon system are 
      susceptible to porcine reproductive and respiratory syndrome virus.
PG  - 383-90
LID - S0166-0934(13)00249-8 [pii]
LID - 10.1016/j.jviromet.2013.06.035 [doi]
AB  - The PiggyBac (PB) transposon system is a non-viral DNA-transfer system in which a 
      transposase directs integration of a PB transposon into a TTAA site in the 
      genome. Transgenic expression of porcine CD163 is necessary and sufficient to 
      confer non-permissive cells susceptible to infection with porcine reproductive 
      and respiratory syndrome virus (PRRSV). Such permissive cells can be used as a 
      tool for PRRSV cellular receptor and other studies. One of the problems in 
      studying PRRSV is the lack of porcine cell lines. In this study, efficient 
      transfection and expression of porcine CD163 in PK-15 cells by PB transposition 
      was demonstrated. The stable PK-15CD163 cell line was used in PRRSV infection 
      assays. The data indicated that the average PB transgene copy number per genome 
      was approximately 10. In line with previous literature the integration of PB into 
      the genome had a bias toward the TTAA chromosomal site. The PK-15CD163 cell line 
      was susceptible to infection by different PRRSV strains and the virus grew to 
      similar titers compared to the Marc-145 cell line. This simplification of 
      PK-15CD163 cell line production will provide a valuable tool to facilitate PRRSV 
      cellular receptor studies and to accelerate existing vectors for PK-15 cell-based 
      gene transfer and expression.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Wang, Xiangpeng
AU  - Wang X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Wei, Ruifang
AU  - Wei R
FAU - Li, Qiongyi
AU  - Li Q
FAU - Liu, Hongliang
AU  - Liu H
FAU - Huang, Baicheng
AU  - Huang B
FAU - Gao, Jiming
AU  - Gao J
FAU - Mu, Yang
AU  - Mu Y
FAU - Wang, Chengbao
AU  - Wang C
FAU - Hsu, Walter H
AU  - Hsu WH
FAU - Hiscox, Julian A
AU  - Hiscox JA
FAU - Zhou, En-Min
AU  - Zhou EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130705
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/*metabolism
MH  - Cell Line
MH  - DNA Transposable Elements
MH  - Gene Expression
MH  - Genomic Instability
MH  - Porcine respiratory and reproductive syndrome virus/growth & 
      development/*physiology
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - Swine
MH  - Transfection/methods
MH  - *Viral Tropism
MH  - Virus Cultivation/methods
MH  - *Virus Internalization
OTO - NOTNLM
OT  - CD163
OT  - DNA-transfer
OT  - PK-15 cell
OT  - PRRSV
OT  - PiggyBac
EDAT- 2013/07/10 06:00
MHDA- 2014/04/20 06:00
CRDT- 2013/07/10 06:00
PHST- 2013/02/20 00:00 [received]
PHST- 2013/06/18 00:00 [revised]
PHST- 2013/06/26 00:00 [accepted]
PHST- 2013/07/10 06:00 [entrez]
PHST- 2013/07/10 06:00 [pubmed]
PHST- 2014/04/20 06:00 [medline]
AID - S0166-0934(13)00249-8 [pii]
AID - 10.1016/j.jviromet.2013.06.035 [doi]
PST - ppublish
SO  - J Virol Methods. 2013 Nov;193(2):383-90. doi: 10.1016/j.jviromet.2013.06.035. 
      Epub 2013 Jul 5.