PMID- 23835134
OWN - NLM
STAT- MEDLINE
DCOM- 20140424
LR  - 20130830
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 94
IP  - 3
DP  - 2013 Sep
TI  - Construction of two ureolytic model organisms for the study of microbially 
      induced calcium carbonate precipitation.
PG  - 290-9
LID - S0167-7012(13)00203-0 [pii]
LID - 10.1016/j.mimet.2013.06.028 [doi]
AB  - Two bacterial strains, Pseudomonas aeruginosa MJK1 and Escherichia coli MJK2, 
      were constructed that both express green fluorescent protein (GFP) and carry out 
      ureolysis. These two novel model organisms are useful for studying bacterial 
      carbonate mineral precipitation processes and specifically ureolysis-driven 
      microbially induced calcium carbonate precipitation (MICP). The strains were 
      constructed by adding plasmid-borne urease genes (ureABC, ureD and ureFG) to the 
      strains P. aeruginosa AH298 and E. coli AF504gfp, both of which already carried 
      unstable GFP derivatives. The ureolytic activities of the two new strains were 
      compared to the common, non-GFP expressing, model organism Sporosarcina pasteurii 
      in planktonic culture under standard laboratory growth conditions. It was found 
      that the engineered strains exhibited a lower ureolysis rate per cell but were 
      able to grow faster and to a higher population density under the conditions of 
      this study. Both engineered strains were successfully grown as biofilms in 
      capillary flow cell reactors and ureolysis-induced calcium carbonate mineral 
      precipitation was observed microscopically. The undisturbed spatiotemporal 
      distribution of biomass and calcium carbonate minerals were successfully resolved 
      in 3D using confocal laser scanning microscopy. Observations of this nature were 
      not possible previously because no obligate urease producer that expresses GFP 
      had been available. Future observations using these organisms will allow 
      researchers to further improve engineered application of MICP as well as study 
      natural mineralization processes in model systems.
CI  - (c) 2013.
FAU - Connolly, James
AU  - Connolly J
AD  - Center for Biofilm Engineering, 366 EPS Building, P.O. Box 173980, Montana State 
      University, Bozeman, MT 59717-3980, USA. james.m.connolly@gmail.com
FAU - Kaufman, Megan
AU  - Kaufman M
FAU - Rothman, Adam
AU  - Rothman A
FAU - Gupta, Rashmi
AU  - Gupta R
FAU - Redden, George
AU  - Redden G
FAU - Schuster, Martin
AU  - Schuster M
FAU - Colwell, Frederick
AU  - Colwell F
FAU - Gerlach, Robin
AU  - Gerlach R
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130705
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Bacterial Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 8W8T17847W (Urea)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Biofilms
MH  - Calcium Carbonate/analysis/*metabolism
MH  - Cloning, Molecular
MH  - Escherichia coli/*genetics/metabolism
MH  - Genetic Engineering
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Microscopy, Confocal
MH  - Models, Biological
MH  - Pseudomonas aeruginosa/*genetics/metabolism
MH  - Sporosarcina/genetics/metabolism
MH  - Urea/metabolism
MH  - Urease/genetics/*metabolism
OTO - NOTNLM
OT  - Calcium carbonate
OT  - Escherichia coli
OT  - GFP
OT  - Pseudomonas aeruginosa
OT  - Sporosarcina pasteurii
OT  - Ureolysis
EDAT- 2013/07/10 06:00
MHDA- 2014/04/25 06:00
CRDT- 2013/07/10 06:00
PHST- 2013/05/20 00:00 [received]
PHST- 2013/06/27 00:00 [revised]
PHST- 2013/06/27 00:00 [accepted]
PHST- 2013/07/10 06:00 [entrez]
PHST- 2013/07/10 06:00 [pubmed]
PHST- 2014/04/25 06:00 [medline]
AID - S0167-7012(13)00203-0 [pii]
AID - 10.1016/j.mimet.2013.06.028 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2013 Sep;94(3):290-9. doi: 10.1016/j.mimet.2013.06.028. Epub 
      2013 Jul 5.