PMID- 23835648
OWN - NLM
STAT- MEDLINE
DCOM- 20140612
LR  - 20181202
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 30
IP  - 3
DP  - 2013 Sep
TI  - Porphyromonas gingivalis lipopolysaccharide increases lipid accumulation by 
      affecting CD36 and ATP-binding cassette transporter A1 in macrophages.
PG  - 1329-36
LID - 10.3892/or.2013.2600 [doi]
AB  - Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) promotes 
      macrophage-derived foam cell formation, however, the mechanisms are not well 
      established. In macrophages, lipid uptake is mediated by scavenger receptors 
      including SR-A and CD36, while the cholesterol efflux is mediated by ATP-binding 
      cassette transporter G1 (ABCG1), ABCA1 and SR-BI. We further investigated the 
      mechanisms underlying the dysregulation by P. gingivalis LPS of these regulators 
      resulting in the promotion of lipid accumulation in THP-1-derived macrophages. 
      Our results showed that P. gingivalis LPS exacerbated lipid accumulation in 
      oxidized low-density lipoprotein (oxLDL)-treated macrophages. However, 
      cholesterol efflux was inhibited by P. gingivalis LPS in THP-1-derived 
      macrophages. In oxLDL-untreated macrophages, P. gingivalis LPS treatment caused 
      an increase in CD36 mRNA and protein levels, and a decrease in ABCA1 mRNA and 
      protein levels, while having no effect on SR-A, SR-BI or ABCG1 expression. 
      Upregulation of CD36 by P. gingivalis LPS resulted from activation of c-Jun/AP-1, 
      and this was confirmed by the inhibition of increased CD36 expression after AP-1 
      inhibition using SP600125. However, the decreased protein stability of ABCA1 by 
      P. gingivalis LPS was a result of increased calpain activity. Moreover, small 
      hairpin RNA (shRNA) targeting heme oxygenase-1 (HO-1) augmented the P. gingivalis 
      LPS-induced atherogenic effects on the expression of c-Jun/AP-1, CD36, ABCA1 and 
      calpain activity. Accordingly, P. gingivalis LPS-regulated promotion of lipid 
      accumulation in foam cells was also exacerbated by HO-1 shRNA. These results 
      indicate that P. gingivalis LPS confers a exacerbation effect on the formation of 
      foam cells by a novel HO-1-dependent mediation of cholesterol efflux and lipid 
      accumulation in macrophages.
FAU - Li, Xiu-Ying
AU  - Li XY
AD  - Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400010, P.R. China.
FAU - Wang, Chun
AU  - Wang C
FAU - Xiang, Xue-Rong
AU  - Xiang XR
FAU - Chen, Fang-Chun
AU  - Chen FC
FAU - Yang, Cong-Ming
AU  - Yang CM
FAU - Wu, Jing
AU  - Wu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130708
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (CD36 Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (oxidized low density lipoprotein)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Blotting, Western
MH  - CD36 Antigens/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cholesterol/*metabolism
MH  - Foam Cells/drug effects
MH  - Heme Oxygenase-1/antagonists & inhibitors/genetics/metabolism
MH  - Humans
MH  - Immunoprecipitation
MH  - Lipid Metabolism/*drug effects
MH  - Lipopolysaccharides/*pharmacology
MH  - Lipoproteins, LDL/pharmacology
MH  - Macrophages/cytology/drug effects/*metabolism
MH  - Porphyromonas gingivalis/*chemistry
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2013/07/10 06:00
MHDA- 2014/06/13 06:00
CRDT- 2013/07/10 06:00
PHST- 2013/05/10 00:00 [received]
PHST- 2013/06/19 00:00 [accepted]
PHST- 2013/07/10 06:00 [entrez]
PHST- 2013/07/10 06:00 [pubmed]
PHST- 2014/06/13 06:00 [medline]
AID - 10.3892/or.2013.2600 [doi]
PST - ppublish
SO  - Oncol Rep. 2013 Sep;30(3):1329-36. doi: 10.3892/or.2013.2600. Epub 2013 Jul 8.