PMID- 23837542
OWN - NLM
STAT- MEDLINE
DCOM- 20131024
LR  - 20130822
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 32
IP  - 9
DP  - 2013 Sep
TI  - Overexpression of TDRP1 gene in swine testis cell and its global transcriptome 
      analysis.
PG  - 511-6
LID - 10.1089/dna.2013.2084 [doi]
AB  - The testis development-related protein-1 (TDRP1) gene was first isolated in 
      spermatogenic cells of testis, and was related to the spermatogenesis and 
      reproduction traits in mammals. This study was performed to further analyze the 
      function of the pig TDRP1 gene by microarray screening of the TDRP1-overexpressed 
      swine testis (ST) cell. After transfection of the recombinant pcDNA3.1-TDRP1 
      vector into the ST cell, the expression of the TDRP1 gene continued to increase 
      at 12, 24, and 36 h post-transfection, and then decreased at 48 h, as indicated 
      by both the fluorescence signal and quantitative PCR. Microarray screening 
      revealed 529 upregulated and 1086 downregulated genes in TDRP1-overexpressed ST 
      cells at 24 h post-transfection (TD-24) versus untransfected control (TD-blank), 
      and 764 upregulated and 858 downregulated genes at 36 h post-transfection (TD-36) 
      versus TD-blank, as well as 720 upregulated and 375 downregulated genes in TD-36 
      versus TD-24. As far as three contrasts were totally considered, 29 common genes 
      were identified, including 17 upregulated genes and 12 downregulated ones. 
      Further, Kyoto encyclopedia of genes and genomes prediction analysis indicated 
      that some of these 29 differently expressed genes were involved in a number of 
      biological pathways. Among the 17 upregulated genes, the PMAIP1 gene was 
      significantly enriched in the p53 signaling pathway, and the others were the 
      DDIT3 gene in the mitogen-activated protein kinases (MAPK) signaling pathway, the 
      PPP1R3C gene in the insulin signaling pathway, as well as the GADD45A gene in the 
      p53 signaling pathway, MAPK signaling pathway, and the cell cycle. Among the 12 
      downregulated genes, the SFRP4 gene was significantly enriched in the Wnt 
      signaling pathway. This study indicated that the TDRP1 gene regulated PMAIP1, 
      GADD45A, DDIT3, and PPP1R3C expression, and these genes involving p53, MAPK, 
      insulin, and Wnt signaling pathways are related to spermatogenesis or 
      reproduction in pig.
FAU - Liu, Manqing
AU  - Liu M
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science, South China Agricultural University, Guangzhou, China.
FAU - Zhang, Wei
AU  - Zhang W
FAU - Luo, Wei
AU  - Luo W
FAU - Nie, Qinghua
AU  - Nie Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130709
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Animals
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Genes, p53
MH  - Genetic Vectors/genetics/metabolism
MH  - MAP Kinase Signaling System
MH  - Male
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Spermatogenesis
MH  - Swine
MH  - Testis/*cytology/metabolism
MH  - Time Factors
MH  - *Transcriptome
MH  - Transfection
MH  - *Up-Regulation
MH  - Wnt Signaling Pathway
EDAT- 2013/07/11 06:00
MHDA- 2013/10/25 06:00
CRDT- 2013/07/11 06:00
PHST- 2013/07/11 06:00 [entrez]
PHST- 2013/07/11 06:00 [pubmed]
PHST- 2013/10/25 06:00 [medline]
AID - 10.1089/dna.2013.2084 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2013 Sep;32(9):511-6. doi: 10.1089/dna.2013.2084. Epub 2013 Jul 9.