PMID- 23839040
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20220129
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 12
IP  - 15
DP  - 2013 Aug 1
TI  - Rapamycin regulates biochemical metabolites.
PG  - 2454-67
LID - 10.4161/cc.25450 [doi]
AB  - The mammalian target of rapamycin (mTOR) kinase is a master regulator of protein 
      synthesis that couples nutrient sensing to cell growth, and deregulation of this 
      pathway is associated with tumorigenesis. p53, and its less investigated family 
      member p73, have been shown to interact closely with mTOR pathways through the 
      transcriptional regulation of different target genes. To investigate the 
      metabolic changes that occur upon inhibition of the mTOR pathway and the role of 
      p73 in this response primary mouse embryonic fibroblast from control and 
      TAp73(-/-) were treated with the macrocyclic lactone rapamycin. Extensive gas 
      chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass 
      spectrometry (LC/MS/MS) analysis were used to obtain a rapamycin-dependent global 
      metabolome profile from control or TAp73(-/-) cells. In total 289 metabolites 
      involved in selective pathways were identified; 39 biochemical metabolites were 
      found to be significantly altered, many of which are known to be associated with 
      the cellular stress response.
FAU - Tucci, Paola
AU  - Tucci P
AD  - Medical Research Council; Toxicology Unit; Leicester, UK; Department of Pharmacy, 
      Health and Nutritional Sciences; University of Calabria; Rende, Cosenza, Italy.
FAU - Porta, Giovanni
AU  - Porta G
FAU - Agostini, Massimiliano
AU  - Agostini M
FAU - Antonov, Alexey
AU  - Antonov A
FAU - Garabadgiu, Alexander Vasilievich
AU  - Garabadgiu AV
FAU - Melino, Gerry
AU  - Melino G
FAU - Willis, Anne E
AU  - Willis AE
LA  - eng
GR  - GGP09133/TI_/Telethon/Italy
GR  - MC_U132670600/MRC_/Medical Research Council/United Kingdom
GR  - MC_UP_A600_1023/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130628
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Nuclear Proteins)
RN  - 0 (delta Np73, mouse)
RN  - AE28F7PNPL (Methionine)
RN  - GAN16C9B8O (Glutathione)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Biosynthetic Pathways/drug effects
MH  - Cells, Cultured
MH  - Glutathione/biosynthesis
MH  - Glycolysis/drug effects
MH  - Metabolome/*drug effects
MH  - Methionine/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Nuclear Proteins/genetics/metabolism
MH  - Pentose Phosphate Pathway/drug effects
MH  - Sirolimus/*pharmacology
PMC - PMC3841324
OTO - NOTNLM
OT  - MEF
OT  - autophagy
OT  - cell death
OT  - mTOR
OT  - metabolism
OT  - p53 family
OT  - p73
OT  - rapamycin
EDAT- 2013/07/11 06:00
MHDA- 2014/04/23 06:00
PMCR- 2013/06/28
CRDT- 2013/07/11 06:00
PHST- 2013/07/11 06:00 [entrez]
PHST- 2013/07/11 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
PHST- 2013/06/28 00:00 [pmc-release]
AID - 25450 [pii]
AID - 2013CC4988R [pii]
AID - 10.4161/cc.25450 [doi]
PST - ppublish
SO  - Cell Cycle. 2013 Aug 1;12(15):2454-67. doi: 10.4161/cc.25450. Epub 2013 Jun 28.