PMID- 23841305
OWN - NLM
STAT- MEDLINE
DCOM- 20150616
LR  - 20181203
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 31
IP  - 3
DP  - 2013 Jun
TI  - [Cytocompatibility of two porous bioactive glass-ceramic in vitro].
PG  - 294-9
AB  - OBJECTIVE: To compare the cytocompatibility of two kinds porous bioactive 
      glass-ceramic made by same raw materials. METHODS: Apatite/wollastonite bioactive 
      glass-ceramic (4006) were prepared by sol-gel method, and bioactive glass (45S5) 
      were prepared by melting method. Bone marrow stromal cells (BMSCs) were 
      cultivated, differentiated and proliferated into osteoblasts, from a rabbit's 
      marrow in the differentiatiofn culture medium with active function. The viability 
      of BMSCs cultivated with extraction of these two kinds of biomaterial, which 
      could represent the cytotoxicity effect of 4006 and 45S5 against BMSCs, was 
      evaluated by the MTp assay. BMSCs were seeded and cocultivated with two kinds of 
      biomaterial scaffolds respectively in vitro. The proliferation and biological 
      properties of cells adhered to scaffolds were observed by inverted phase contrast 
      microscope, scanning electron microscope (SEM), and environmental scanning 
      electron microscope (ESEM), and a suitable cell amount for seeding on the 
      scaffold was searched. RESULTS: There was no difference on the viability of BMSCs 
      only cultured for one day by complete extract of 4006 and culture medium 
      (P>0.05), but there was significant difference between them when the cells had 
      been cultured for 3 days(P<0.01). The extract of 45S5 had significantly higher 
      cytotoxicity than extract of culture medium (P<0.01). The BMSCs adhered, spread, 
      and proliferated throughout the pores of the scaffold 4006, and the amount of 
      cells adhered to 4006 was more than to 45S5. The adhered cells to 4006 increased 
      with the rising amount of cells seeded. And 2 x 10(7) cells.mL-1 suspension 
      resulted inthe highest cell adherence during the comparative cells adherence 
      test. CONCLUSION: Apatite/woolastonite bioac tive glass-ceramic has good 
      bioactivity and cytocompatibility. Therefore, it may have the potential to be a 
      new cell vehicle for bone tissue engineering. And the suitable seeding cell 
      amount of apatite/wollastonite bioactive glass-ceramic should be 2x10(7) 
      cells.mL-1 or even more than that.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Tongji 
      University, Shanghai, China.
FAU - Jiang, Xinquan
AU  - Jiang X
FAU - Zhang, Xiuli
AU  - Zhang X
FAU - Wang, Deping
AU  - Wang D
FAU - Zhen, Lei
AU  - Zhen L
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Compounds)
RN  - 0 (Silicates)
RN  - 85422-94-2 (Glass ceramics)
RN  - S4255P4G5M (calcium silicate)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Calcium Compounds
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Ceramics
MH  - Glass
MH  - In Vitro Techniques
MH  - Mesenchymal Stem Cells
MH  - Osteoblasts
MH  - Rabbits
MH  - Silicates
MH  - *Tissue Engineering
EDAT- 2013/07/12 06:00
MHDA- 2015/06/17 06:00
CRDT- 2013/07/12 06:00
PHST- 2013/07/12 06:00 [entrez]
PHST- 2013/07/12 06:00 [pubmed]
PHST- 2015/06/17 06:00 [medline]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2013 Jun;31(3):294-9.