PMID- 23841781
OWN - NLM
STAT- MEDLINE
DCOM- 20140228
LR  - 20130711
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 121
IP  - 4
DP  - 2013 Aug
TI  - Expression of Clu and Tgfb1 during murine tooth development: effects of in-vivo 
      transfection with anti-miR-214.
PG  - 303-12
LID - 10.1111/eos.12056 [doi]
AB  - Expression of clusterin (Clu) in the murine first molar tooth germ was markedly 
      increased at postnatal developmental stages. The time-course of expression of 
      this gene paralleled those of other genes encoding proteins involved during the 
      secretory phase of odontogenesis, as described previously. Immunohistochemical 
      studies of clusterin in murine molar tooth germs suggested this protein to be 
      located in outer enamel epithelium, regressing enamel organ, secretory 
      ameloblasts, and the dental epithelium connecting the tooth to the oral 
      epithelium at an early eruptive stage. Immunolabelling of transforming growth 
      factor beta-1 (TGF-beta1) revealed it to be located close to clusterin. The levels 
      of expression of Clu and Tgfb1 were markedly decreased following in-vivo 
      transfection with anti-miR-214. In contrast, the expression of several genes 
      associated with regulation of growth and development were increased by this 
      treatment. We suggest that clusterin has functions during secretory odontogenesis 
      and the early eruptive phase. Bioinformatic analysis after treatment with 
      anti-miR-214 suggested that, whilst cellular activities associated with tooth 
      mineralization and eruption were inhibited, activities associated with an 
      alternative developmental activity (i.e. biosynthesis of contractile proteins) 
      appeared to be stimulated. These changes probably occur through regulation 
      mediated by a common cluster of transcription factors and support suggestions 
      that microRNAs (miRNAs) are highly significant as regulators of differentiation 
      during odontogenesis.
CI  - (c) 2013 Eur J Oral Sci.
FAU - Khan, Qalb-E-Saleem
AU  - Khan QE
AD  - Department of Oral Biology, University of Oslo, Oslo, Norway. 
      qsahmed@odont.uio.no
FAU - Sehic, Amer
AU  - Sehic A
FAU - Khuu, Cuong
AU  - Khuu C
FAU - Risnes, Steinar
AU  - Risnes S
FAU - Osmundsen, Harald
AU  - Osmundsen H
LA  - eng
PT  - Journal Article
DEP - 20130528
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Clusterin)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn214 microRNA, mouse)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Clusterin/analysis/*genetics
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Developmental
MH  - Immunohistochemistry
MH  - Mice
MH  - MicroRNAs/*antagonists & inhibitors
MH  - Molar/embryology/*growth & development
MH  - Odontogenesis/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tooth Calcification/*genetics
MH  - Tooth Germ/embryology/*growth & development/metabolism
MH  - Transfection
MH  - Transforming Growth Factor beta1/analysis/*genetics
OTO - NOTNLM
OT  - clusterin
OT  - eruption
OT  - miR-214
OT  - mouse molar
OT  - transforming growth factor beta-1
EDAT- 2013/07/12 06:00
MHDA- 2014/03/01 06:00
CRDT- 2013/07/12 06:00
PHST- 2013/04/11 00:00 [accepted]
PHST- 2013/07/12 06:00 [entrez]
PHST- 2013/07/12 06:00 [pubmed]
PHST- 2014/03/01 06:00 [medline]
AID - 10.1111/eos.12056 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2013 Aug;121(4):303-12. doi: 10.1111/eos.12056. Epub 2013 May 28.