PMID- 23841891
OWN - NLM
STAT- MEDLINE
DCOM- 20131210
LR  - 20230124
IS  - 1600-6143 (Electronic)
IS  - 1600-6135 (Linking)
VI  - 13
IP  - 8
DP  - 2013 Aug
TI  - Influence of test technique on sensitization status of patients on the kidney 
      transplant waiting list.
PG  - 2075-82
LID - 10.1111/ajt.12332 [doi]
AB  - The exquisitely sensitive single antigen bead (SAB) technique was shown to detect 
      human leukocyte antigen (HLA) antibodies in sera of healthy male blood donors. 
      Such false reactions can have an impact on critical decisions, especially with 
      respect to the determination of unacceptable HLA-antigen mismatches in patients 
      awaiting a kidney transplant. We tested pretransplant sera of 534 patients on the 
      kidney waiting list using complement-dependent cytotoxicity (CDC), enzyme-linked 
      immunosorbent assay (ELISA) and SAB in parallel. Evidence of HLA antibodies was 
      obtained in 5% of patients using CDC, 14% using ELISA, and 81% using SAB. Among 
      patients without history of an immunizing event, 77% showed evidence of HLA 
      antibodies in SAB. In contrast 98% of these patients were negative in ELISA and 
      CDC. In patients without an immunizing event, SAB-detected antibodies reacted not 
      always weakly but with mean fluorescence intensity (MFI) values as high as 14 
      440. High-MFI-value antibodies were found in some of these patients with HLA 
      specificities that are rather common in general population, consideration of 
      which would lead to unjustified exclusion of potential kidney donors. False SAB 
      reactions can be unveiled by testing with additional antibody assays. Denial of 
      donor kidneys to recipients based on HLA-antibody specificities detected 
      exclusively in the SAB assay is not advisable.
CI  - (c) Copyright 2013 The American Society of Transplantation and the American Society 
      of Transplant Surgeons.
FAU - Gombos, P
AU  - Gombos P
AD  - Department of Transplantation Immunology, University of Heidelberg, Heidelberg, 
      Germany.
FAU - Opelz, G
AU  - Opelz G
FAU - Scherer, S
AU  - Scherer S
FAU - Morath, C
AU  - Morath C
FAU - Zeier, M
AU  - Zeier M
FAU - Schemmer, P
AU  - Schemmer P
FAU - Susal, C
AU  - Susal C
LA  - eng
PT  - Journal Article
DEP - 20130710
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
CIN - Am J Transplant. 2013 Aug;13(8):1951-2. PMID: 23837443
CIN - Am J Transplant. 2014 Jan;14(1):236. PMID: 24279946
MH  - Antibody Specificity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - HLA Antigens/*immunology
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/blood/*immunology
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Tissue Donors
MH  - Waiting Lists
OTO - NOTNLM
OT  - Antibody screening
OT  - HLA antibodies
OT  - kidney transplantation
OT  - single antigen beads
OT  - unacceptable HLA antigen mismatches
EDAT- 2013/07/12 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/07/12 06:00
PHST- 2012/11/07 00:00 [received]
PHST- 2013/04/05 00:00 [revised]
PHST- 2013/04/08 00:00 [accepted]
PHST- 2013/07/12 06:00 [entrez]
PHST- 2013/07/12 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - S1600-6135(22)26059-6 [pii]
AID - 10.1111/ajt.12332 [doi]
PST - ppublish
SO  - Am J Transplant. 2013 Aug;13(8):2075-82. doi: 10.1111/ajt.12332. Epub 2013 Jul 
      10.