PMID- 23842788
OWN - NLM
STAT- MEDLINE
DCOM- 20140423
LR  - 20211021
IS  - 1432-1327 (Electronic)
IS  - 0949-8257 (Print)
IS  - 0949-8257 (Linking)
VI  - 18
IP  - 7
DP  - 2013 Oct
TI  - Dual effect of heparin on Fe(2)(+)-induced cardiolipin peroxidation: implications for 
      peroxidation of cytochrome c oxidase bound cardiolipin.
PG  - 729-37
LID - 10.1007/s00775-013-1019-z [doi]
AB  - The effect of heparin on peroxidation of cardiolipin (CL) initiated by ferrous 
      iron was studied in vitro using detergent-solubilized CL, liposomal CL, or CL 
      bound to isolated cytochrome c oxidase (CcO). Heparin increased both the rate and 
      the extent of CL peroxidation for detergent-solubilized CL and for CcO-bound CL. 
      The effect of heparin was time- and concentration-dependent as monitored by the 
      formation of conjugated dienes or thiobarbituric acid reactive substances. The 
      results showed great similarity between the effect of heparin and the effect of 
      certain iron chelators, such as ADP, on phospholipid peroxidation. Heparin 
      increased the peroxidation of CcO-bound CL only when tertiary butyl hydroperoxide 
      was also present. The enzyme activity of the resulting CcO complex decreased 25 
      %, in part due to peroxidation of functionally important CL. In contrast to 
      peroxidation of detergent-solubilized CL, peroxidation of liposomal CL was 
      inhibited by heparin, suggesting that the effect of heparin and ferrous iron 
      depends on their proximity to the acyl chains of CL.
FAU - Musatov, Andrej
AU  - Musatov A
AD  - Department of Biochemistry, The University of Texas Health Science Center at San 
      Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229-3900, USA, 
      musatov@saske.sk.
LA  - eng
GR  - R01 GM024795/GM/NIGMS NIH HHS/United States
GR  - GM024795/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130711
PL  - Germany
TA  - J Biol Inorg Chem
JT  - Journal of biological inorganic chemistry : JBIC : a publication of the Society 
      of Biological Inorganic Chemistry
JID - 9616326
RN  - 0 (Cardiolipins)
RN  - 0 (Detergents)
RN  - 0 (Liposomes)
RN  - 9005-49-6 (Heparin)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cardiolipins/chemistry/*metabolism
MH  - Cattle
MH  - Detergents/chemistry
MH  - Electron Transport Complex IV/chemistry/*metabolism
MH  - Heparin/*pharmacology
MH  - Iron/*pharmacology
MH  - Lipid Peroxidation/*drug effects
MH  - Liposomes/metabolism
MH  - Solubility
PMC - PMC3783530
MID - NIHMS504717
EDAT- 2013/07/12 06:00
MHDA- 2014/04/24 06:00
PMCR- 2014/10/01
CRDT- 2013/07/12 06:00
PHST- 2013/01/31 00:00 [received]
PHST- 2013/06/22 00:00 [accepted]
PHST- 2013/07/12 06:00 [entrez]
PHST- 2013/07/12 06:00 [pubmed]
PHST- 2014/04/24 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - 10.1007/s00775-013-1019-z [doi]
PST - ppublish
SO  - J Biol Inorg Chem. 2013 Oct;18(7):729-37. doi: 10.1007/s00775-013-1019-z. Epub 
      2013 Jul 11.