PMID- 23842936
OWN - NLM
STAT- MEDLINE
DCOM- 20140114
LR  - 20191027
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 28
IP  - 7
DP  - 2013 Jul
TI  - Role of protein kinase G on the post-shock mesenteric lymph blockage ameliorating 
      vascular calcium sensitivity.
PG  - 537-42
LID - S0102-86502013000700010 [pii]
AB  - PURPOSE: To investigate the role of protein kinase G (PKG) in blocking post-shock 
      mesenteric lymph (PSML) return ameliorating the calcium sensitivity in 
      hemorrhagic shock rats. METHODS: Male Wistar rats were randomly divided into 
      sham, shock, shock+ligation (shock plus mesenteric lymph duct ligation (MLDL)), 
      shock+drainage (shock plus PSML drainage) groups. After shock (hypotension 40 mm 
      Hg) for three hours or corresponding times, the superior mesenteric artery (SMA) 
      was taken out for detecting the PKG and phospho PKG (p-PKG) contents, and the 
      vascular rings of SMA were prepared for assaying the calcium sensitivity using an 
      isolated organ perfusion system. RESULTS: The PKG and p-PKG contents of SMA in 
      shock group were significantly increased than that of sham group, and MLDL or 
      PSML drainage reducing the levels of PKG and p-PKG. Meanwhile, the vascular 
      calcium sensitivity in shock group was significantly lower than that of sham 
      group, MLDL or PSML drainage enhanced the calcium sensitivity. After incubating 
      with PKG regulators in shock+ligation and shock+drainage groups, the PKG agonist 
      8Br-cGMP reduced the contractility of vascular rings to gradient calcium ions and 
      Emax and the PKG inhibitor agonist KT5823 elevated the calcium sensitivity 
      significantly. CONCLUSION: Protein kinase G plays an important role in post-shock 
      mesenteric lymph blockage improving vascular calcium sensitivity.
FAU - Zhao, Zi-Gang
AU  - Zhao ZG
AD  - Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
FAU - Wei, Yan-Ling
AU  - Wei YL
FAU - Niu, Chun-Yu
AU  - Niu CY
FAU - Zhang, Yu-Ping
AU  - Zhang YP
FAU - Zhang, Li-Min
AU  - Zhang LM
FAU - Jiang, Li-Na
AU  - Jiang LN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Calcium/analysis/*metabolism
MH  - Cyclic GMP-Dependent Protein Kinases/analysis/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Male
MH  - Mesenteric Artery, Superior/*metabolism/physiopathology
MH  - Muscle Contraction
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Shock, Hemorrhagic/*metabolism/physiopathology
EDAT- 2013/07/12 06:00
MHDA- 2014/01/15 06:00
CRDT- 2013/07/12 06:00
PHST- 2013/03/25 00:00 [received]
PHST- 2013/06/24 00:00 [accepted]
PHST- 2013/07/12 06:00 [entrez]
PHST- 2013/07/12 06:00 [pubmed]
PHST- 2014/01/15 06:00 [medline]
AID - S0102-86502013000700010 [pii]
AID - 10.1590/s0102-86502013000700010 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2013 Jul;28(7):537-42. doi: 10.1590/s0102-86502013000700010.