PMID- 2384346
OWN - NLM
STAT- MEDLINE
DCOM- 19900914
LR  - 20071114
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 11
IP  - 3
DP  - 1990 May-Jun
TI  - Studies on peroxisomes of the adult rat Leydig cell.
PG  - 270-8
AB  - The aims of this study were to differentially identify peroxisomes and lysosomes 
      in Leydig cells of the sexually mature rat using cytochemical techniques, to 
      describe the size and shape of peroxisomal profiles, and to localize catalase and 
      sterol carrier protein-2 (SCP2) in Leydig cell peroxisomes. Peroxisome profiles, 
      identified by cytochemical staining for catalase activity using 
      3,3'-diaminobenzidine tetrahydrochloride (DAB) were categorized according to 
      their longest diameter as small (less than 0.18 microns), intermediate (0.18-0.45 
      microns), and large (more than 0.45 microns); and according to their shape, which 
      were designated as circular, oval, and dumbbell. Together these peroxisomal 
      profiles occupied 11.2 microns 3/Leydig cell. Lysosomes, identified in the same 
      tissue sections as acid phosphatase positive organelles, occupied 12.9 microns 
      3/Leydig cell. Negative bodies with morphology identical to cytochemically 
      unstained peroxisomes also were detected. These organelles occupied 14.5 microns 
      3/Leydig cell. Catalase was immunolocalized exclusively in Leydig cell 
      peroxisomes using AuroProbe EM protein A G10 (ie, 10 nm gold particles). Sterol 
      carrier protein-2 was immunolocalized in Leydig cell peroxisomes by AuroProbe EM 
      protein A G15 (ie, 15 nm gold particles). Immunolocalization of catalase and SCP2 
      using 10 nm and 15 nm gold particles in the same peroxisomes confirmed that 
      Leydig cell peroxisomes contain SCP2. Taken together, these results show 
      conclusively that adult rat Leydig cell peroxisomal profiles occur in different 
      shapes and sizes, which suggests the existence of a network of peroxisomes, 
      rather than peroxisomes occurring as separate isolated organelles. More 
      importantly, the present study demonstrates for the first time that Leydig cell 
      peroxisomes contain SCP2.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Mendis-Handagama, S M
AU  - Mendis-Handagama SM
AD  - Department of Population Dynamics, John Hopkins School of Hygiene and Public 
      Health, Baltimore, Maryland 21205.
FAU - Zirkin, B R
AU  - Zirkin BR
FAU - Scallen, T J
AU  - Scallen TJ
FAU - Ewing, L L
AU  - Ewing LL
LA  - eng
GR  - HD07204/HD/NICHD NIH HHS/United States
GR  - P-30 HD06208/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (sterol carrier proteins)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/metabolism
MH  - Catalase/metabolism
MH  - Leydig Cells/*ultrastructure
MH  - Lysosomes/metabolism/ultrastructure
MH  - Male
MH  - Microbodies/metabolism/*ultrastructure
MH  - *Plant Proteins
MH  - Random Allocation
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
PST - ppublish
SO  - J Androl. 1990 May-Jun;11(3):270-8.