PMID- 23844583
OWN - NLM
STAT- MEDLINE
DCOM- 20140324
LR  - 20181202
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 51
IP  - 10
DP  - 2013 Oct
TI  - Anti-HIV-1 integrase compound from Pometia pinnata leaves.
PG  - 1256-61
LID - 10.3109/13880209.2013.786098 [doi]
AB  - CONTEXT: HIV-1 integrase (HIV-1 IN) is a key enzyme involved in the replication 
      cycle of the retrovirus. Any new knowledge on inhibitors of this enzyme could 
      provide essential clues for the development of anti-HIV drugs. OBJECTIVE: To 
      evaluate anti-HIV-1 IN activity of some Thai medicinal plant extracts, and the 
      extract that possessed the strongest anti-HIV-1 IN activity was subjected to 
      isolation of the active compounds. MATERIALS AND METHODS: Ethanol extracts of 
      eight Thai medicinal plants were evaluated for their inhibitory effect against 
      HIV-1 IN. An extract of Pometia pinnata J. R. Forst. & G. Forst (Sapindaceae) 
      leaves that possessed the strongest anti-HIV-1 IN activity was fractionated to 
      isolate the active compounds by anti-HIV-1 IN assay-guided isolation process. 
      RESULTS AND DISCUSSION: The leaf extract from P. pinnata had the strongest 
      anti-HIV-1 IN activity with an IC50 value of 8.8 microg/mL. An anti-HIV-1 IN 
      assay-guided isolation of the active compounds from a leaf extract of P. pinnata 
      resulted in the isolation of one active compound, identified as proanthocyanidin 
      A2. Proanthocyanidin A2 showed satisfactory anti-HIV-1 IN activity with an IC50 
      value of 30.1 microM. Three flavonoids, epicatechin, kaempferol-3-O-rhamnoside, 
      quercetin-3-O-rhamnoside; a glycolipid, 
      1-O-palmitoyl-3-O-[alpha-.-galactopyranosyl-(1 --> 6)-beta-.-galactopyranosyl]-sn-glycerol; 
      a steroidal glycoside; stigmasterol-3-O-glucoside; and a pentacyclic triterpenoid 
      saponin, 
      3-O-alpha-.-arabinofuranosyl-(1 --> 3)-[alpha-.-rhamnopyranosyl-(1 --> 2)]-alpha-.-arabinopyranosyl 
      hederagenin were also isolated but were inactive at a concentration of 100 microM.
FAU - Suedee, Areerat
AU  - Suedee A
AD  - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical 
      Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
FAU - Tewtrakul, Supinya
AU  - Tewtrakul S
FAU - Panichayupakaranant, Pharkphoom
AU  - Panichayupakaranant P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130711
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (HIV Integrase Inhibitors)
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.7.- (HIV Integrase)
RN  - YY6481J2FF (p31 integrase protein, Human immunodeficiency virus 1)
SB  - IM
MH  - Chemical Fractionation
MH  - Ethanol/chemistry
MH  - HIV Integrase/*metabolism
MH  - HIV Integrase Inhibitors/chemistry/isolation & purification/*pharmacology
MH  - Phytotherapy
MH  - Plant Extracts/chemistry/isolation & purification/*pharmacology
MH  - Plant Leaves
MH  - Plants, Medicinal
MH  - *Sapindaceae/chemistry
MH  - Solvents/chemistry
EDAT- 2013/07/13 06:00
MHDA- 2014/03/25 06:00
CRDT- 2013/07/13 06:00
PHST- 2013/07/13 06:00 [entrez]
PHST- 2013/07/13 06:00 [pubmed]
PHST- 2014/03/25 06:00 [medline]
AID - 10.3109/13880209.2013.786098 [doi]
PST - ppublish
SO  - Pharm Biol. 2013 Oct;51(10):1256-61. doi: 10.3109/13880209.2013.786098. Epub 2013 
      Jul 11.