PMID- 23845229
OWN - NLM
STAT- MEDLINE
DCOM- 20140424
LR  - 20220317
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 94
IP  - 3
DP  - 2013 Sep
TI  - Rapid and reliable MALDI-TOF mass spectrometry identification of Candida 
      non-albicans isolates from bloodstream infections.
PG  - 262-6
LID - S0167-7012(13)00205-4 [pii]
LID - 10.1016/j.mimet.2013.07.001 [doi]
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry (MS) fingerprinting has recently become an effective instrument for 
      rapid microbiological diagnostics and in particular for identification of 
      micro-organisms directly in a positive blood culture. The aim of the study was to 
      evaluate a collection of 82 stored yeast isolates from bloodstream infection, by 
      MALDI-TOF MS; 21 isolates were identified also directly from positive blood 
      cultures and in the presence of other co-infecting micro-organisms. Of the 82 
      isolates grown on plates, 64 (76%) were correctly identified by the Vitek II 
      system and 82 (100%) by MALDI-TOF MS; when the two methods gave different 
      results, the isolate was identified by PCR. MALDI-TOF MS was unreliable in 
      identifying two isolates (Candida glabrata and Candida parapsilosis) directly 
      from blood culture; however, direct analysis from positive blood culture samples 
      was fast and effective for the identification of yeast, which is of great 
      importance for early and adequate treatment.
CI  - (c) 2013. Published by Elsevier B.V. All rights reserved.
FAU - Pulcrano, Giovanna
AU  - Pulcrano G
AD  - Department of Molecular Medicine and Medical Biotechnology, Medicine School, 
      University of Naples Federico II, via Pansini, 80131, Naples, Italy. 
      giovannapulcrano@libero.it
FAU - Iula, Dora Vita
AU  - Iula DV
FAU - Vollaro, Antonio
AU  - Vollaro A
FAU - Tucci, Alessandra
AU  - Tucci A
FAU - Cerullo, Monica
AU  - Cerullo M
FAU - Esposito, Matilde
AU  - Esposito M
FAU - Rossano, Fabio
AU  - Rossano F
FAU - Catania, Maria Rosaria
AU  - Catania MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130709
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
SB  - IM
MH  - *Candida/chemistry/classification/isolation & purification
MH  - Candidemia/*microbiology
MH  - Cluster Analysis
MH  - Humans
MH  - Mycological Typing Techniques/*methods
MH  - Phylogeny
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
OTO - NOTNLM
OT  - BC
OT  - BSI
OT  - Bloodstream infections
OT  - Candida non-albicans
OT  - ICU
OT  - ITS
OT  - MALDI-TOF
OT  - Vitek II
OT  - blood culture
OT  - bloodstream infection
OT  - intensive care unit
OT  - internal transcribed spacer
OT  - matrix-assisted laser desorption/ionization time-of-flight
EDAT- 2013/07/13 06:00
MHDA- 2014/04/25 06:00
CRDT- 2013/07/13 06:00
PHST- 2013/05/12 00:00 [received]
PHST- 2013/06/30 00:00 [revised]
PHST- 2013/07/01 00:00 [accepted]
PHST- 2013/07/13 06:00 [entrez]
PHST- 2013/07/13 06:00 [pubmed]
PHST- 2014/04/25 06:00 [medline]
AID - S0167-7012(13)00205-4 [pii]
AID - 10.1016/j.mimet.2013.07.001 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2013 Sep;94(3):262-6. doi: 10.1016/j.mimet.2013.07.001. Epub 
      2013 Jul 9.