PMID- 23845800
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20211021
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 31
IP  - 38
DP  - 2013 Aug 28
TI  - A West Nile virus NS4B-P38G mutant strain induces adaptive immunity via 
      TLR7-MyD88-dependent and independent signaling pathways.
PG  - 4143-51
LID - S0264-410X(13)00903-1 [pii]
LID - 10.1016/j.vaccine.2013.06.093 [doi]
AB  - Prior work shows that an attenuated West Nile virus (WNV), the nonstructural 
      (NS)4B-P38G mutant infection in mice induced strong immune responses and 
      protected host from subsequent lethal wild-type WNV infection. Here, we 
      investigated NS4B-P38G mutant infection in myeloid differentiation factor 
      88-deficient (MyD88(-/-)) and Toll-like receptor 7-deficient (TLR7(-/-)) mice and 
      found they had enhanced susceptibility compared to wild-type mice. Both groups 
      had lower WNV-specific IgM response and reduced effector T cell functions. 
      Dendritic cells (DCs) also exhibited a reduced maturation and impaired 
      antigen-presenting functions compared to wild-type DCs. Moreover, infection with 
      NS4B-P38G mutant in TLR7(-/-) and MyD88(-/-) mice provided full and partial 
      protection respectively from subsequent challenge with lethal wild-type WNV. 
      There were reduced T cell responses in MyD88(-/-) and interleukin-1 receptor 
      deficient (IL-1R(-/-)) mice during secondary challenge with wild-type WNV. In 
      contrast, TLR7(-/-) mice displayed normal T cell functions. Collectively, these 
      results suggest that TLR7-dependent MyD88 signaling is required for T cell 
      priming during NS4B-P38G mutant infection, whereas the TLR7-independent MyD88 
      signaling pathways are involved in memory T cell development, which may 
      contribute to host protection during secondary challenge with wild-type WNV.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Xie, Guorui
AU  - Xie G
AD  - Department of Microbiology & Immunology, The University of Texas Medical Branch, 
      Galveston, TX 77555, USA.
FAU - Welte, Thomas
AU  - Welte T
FAU - Wang, Jia
AU  - Wang J
FAU - Whiteman, Melissa C
AU  - Whiteman MC
FAU - Wicker, Jason A
AU  - Wicker JA
FAU - Saxena, Vandana
AU  - Saxena V
FAU - Cong, Yingzi
AU  - Cong Y
FAU - Barrett, Alan D T
AU  - Barrett AD
FAU - Wang, Tian
AU  - Wang T
LA  - eng
GR  - R01 AI072060/AI/NIAID NIH HHS/United States
GR  - AI072060/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130708
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Cytokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Interleukin-1)
RN  - 0 (Tlr7 protein, mouse)
RN  - 0 (Toll-Like Receptor 7)
SB  - IM
MH  - *Adaptive Immunity
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cytokines/metabolism
MH  - Dendritic Cells/immunology/virology
MH  - Host-Pathogen Interactions
MH  - Immunity, Humoral
MH  - Immunologic Memory
MH  - Membrane Glycoproteins/genetics/immunology/*metabolism
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Myeloid Differentiation Factor 88/genetics/immunology/*metabolism
MH  - Receptors, Interleukin-1/genetics/metabolism
MH  - Signal Transduction/immunology
MH  - T-Lymphocytes/immunology/metabolism/virology
MH  - Toll-Like Receptor 7/genetics/immunology/*metabolism
MH  - West Nile virus/*genetics/*immunology/pathogenicity
PMC - PMC3870894
MID - NIHMS502830
OTO - NOTNLM
OT  - B6
OT  - BM
OT  - C57BL/6
OT  - CNS
OT  - DCs
OT  - E
OT  - IFN
OT  - Immune response
OT  - LD
OT  - MyD88
OT  - NS
OT  - NS4B protein
OT  - Q-PCR
OT  - T cell
OT  - TLR
OT  - Toll-like receptor
OT  - WNV
OT  - West Nile virus
OT  - bone marrow
OT  - central nervous system
OT  - dendritic cells
OT  - envelope
OT  - i.p.
OT  - interferon
OT  - intraperitoneally
OT  - lethal dose
OT  - myeloid differentiation factor 88
OT  - nonstructural
OT  - quantitative PCR
EDAT- 2013/07/13 06:00
MHDA- 2014/08/27 06:00
PMCR- 2014/08/28
CRDT- 2013/07/13 06:00
PHST- 2013/02/14 00:00 [received]
PHST- 2013/06/20 00:00 [revised]
PHST- 2013/06/25 00:00 [accepted]
PHST- 2013/07/13 06:00 [entrez]
PHST- 2013/07/13 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
PHST- 2014/08/28 00:00 [pmc-release]
AID - S0264-410X(13)00903-1 [pii]
AID - 10.1016/j.vaccine.2013.06.093 [doi]
PST - ppublish
SO  - Vaccine. 2013 Aug 28;31(38):4143-51. doi: 10.1016/j.vaccine.2013.06.093. Epub 
      2013 Jul 8.