PMID- 23845887
OWN - NLM
STAT- MEDLINE
DCOM- 20150421
LR  - 20140709
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 52
IP  - 7
DP  - 2014 Aug
TI  - A sensitive and validated HPLC method for the determination of cyromazine and 
      melamine in herbal and edible plants using accelerated solvent extraction and 
      cleanup with SPE.
PG  - 751-7
LID - 10.1093/chromsci/bmt089 [doi]
AB  - A highly sensitive method was developed for the determination of the residues of 
      cyromazine (CYR) and its metabolite, melamine (MEL), in herbal and edible plant 
      samples by using reversed phase high-performance liquid 
      chromatography-diode-array detection (RP-HPLC-DAD) with accelerated solvent 
      extraction and solid phase extraction cleanup. The conditions of separation and 
      detection were investigated and optimized. A Waters C18 column (250 x 4.6 mm 
      i.d., 5 microm) was used for the RP-HPLC, with a mobile phase composed of 0.1% 
      trifluoroacetic acid solution and methanol (85:15, v/v, pH 2.6). Under the 
      optimized conditions, good linearity was achieved with a correlation coefficient 
      of 0.9998. The limits of quantification of the method were 2.15 microg/kg for CYR and 
      2.51 microg/kg for MEL, which are maximum residue limits as low as three orders of 
      magnitude. The recovery values at three spiked concentrations were in the range 
      of 96.2-107.1% with relative standard deviations (RSDs) of 1.1-5.7% for CYR, and 
      92.7-104.9% with RSDs of 1.7-6.1% for MEL. The proposed method allows detection 
      at levels as low as microg/kg levels for CYR and MEL. The method was validated by 
      liquid chromatography-tandem mass spectrometry, and can be used for the routine 
      determination of CYR and MEL in herbal and edible plant samples with the 
      characteristics of speed, high sensitivity and accuracy, and low consumption of 
      reagents.
CI  - (c) The Author [2013]. Published by Oxford University Press. All rights reserved. 
      For Permissions, please email: journals.permissions@oup.com.
FAU - Ge, Xusheng
AU  - Ge X
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology of Hebei Province, Wusi East Road 180, 
      Baoding, 071002, P. R. China Biochemical Department, Baoding University, Qiyi 
      East Road 3027, Baoding, 07100, P. R. China.
FAU - Wu, Xingqiang
AU  - Wu X
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology of Hebei Province, Wusi East Road 180, 
      Baoding, 071002, P. R. China.
FAU - Liang, Shuxuan
AU  - Liang S
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology of Hebei Province, Wusi East Road 180, 
      Baoding, 071002, P. R. China.
FAU - Sun, Hanwen
AU  - Sun H
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology of Hebei Province, Wusi East Road 180, 
      Baoding, 071002, P. R. China gexusheng@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130711
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Triazines)
RN  - CA49Y29RA9 (cyromazine)
RN  - N3GP2YSD88 (melamine)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Least-Squares Analysis
MH  - Plants, Edible/*chemistry
MH  - Plants, Medicinal/*chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/*methods
MH  - Triazines/*analysis
EDAT- 2013/07/13 06:00
MHDA- 2015/04/22 06:00
CRDT- 2013/07/13 06:00
PHST- 2013/07/13 06:00 [entrez]
PHST- 2013/07/13 06:00 [pubmed]
PHST- 2015/04/22 06:00 [medline]
AID - bmt089 [pii]
AID - 10.1093/chromsci/bmt089 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2014 Aug;52(7):751-7. doi: 10.1093/chromsci/bmt089. Epub 2013 
      Jul 11.