PMID- 23848839
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20211203
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Print)
IS  - 0002-7863 (Linking)
VI  - 135
IP  - 30
DP  - 2013 Jul 31
TI  - Molybdopterin biosynthesis: trapping an unusual purine ribose adduct in the 
      MoaA-catalyzed reaction.
PG  - 10883-5
LID - 10.1021/ja4041048 [doi]
AB  - MoaA/MoaC catalyze a remarkable rearrangement reaction in which 
      guanosine-5'-triphosphate (GTP) is converted to cyclic pyranopterin monophosphate 
      (cPMP). In this reaction, the C8 of GTP is inserted between the C2' and the C3' 
      carbons of the GTP ribose. Previous experiments with GTP isotopomers demonstrated 
      that the ribose C3' hydrogen atom is abstracted by the adenosyl radical. This led 
      to a novel mechanistic proposal involving an intermediate with a bond between the 
      C8 of guanine and C3' of the ribose. This paper describes the use of 
      2',3'-dideoxyGTP to trap this intermediate.
FAU - Mehta, Angad P
AU  - Mehta AP
AD  - Department of Chemistry, Texas A&M University, College Station, Texas 77842, USA.
FAU - Abdelwahed, Sameh H
AU  - Abdelwahed SH
FAU - Begley, Tadhg P
AU  - Begley TP
LA  - eng
GR  - R01 DK044083/DK/NIDDK NIH HHS/United States
GR  - R37 DK044083/DK/NIDDK NIH HHS/United States
GR  - DK44083/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130722
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Coenzymes)
RN  - 0 (Metalloproteins)
RN  - 0 (Molybdenum Cofactors)
RN  - 0 (Pteridines)
RN  - 681HV46001 (Ribose)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - ATN6EG42UQ (molybdenum cofactor)
RN  - EC 3.- (Hydrolases)
SB  - IM
MH  - *Biocatalysis
MH  - Coenzymes/*biosynthesis
MH  - Guanosine Triphosphate/*chemistry
MH  - Hydrolases/*metabolism
MH  - Metalloproteins/*biosynthesis
MH  - Molybdenum Cofactors
MH  - Pteridines
MH  - Ribose/*chemistry/*metabolism
PMC - PMC3804160
MID - NIHMS508897
EDAT- 2013/07/16 06:00
MHDA- 2014/02/19 06:00
PMCR- 2014/07/31
CRDT- 2013/07/16 06:00
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
PHST- 2014/07/31 00:00 [pmc-release]
AID - 10.1021/ja4041048 [doi]
PST - ppublish
SO  - J Am Chem Soc. 2013 Jul 31;135(30):10883-5. doi: 10.1021/ja4041048. Epub 2013 Jul 
      22.