PMID- 23849069 OWN - NLM STAT- MEDLINE DCOM- 20140127 LR - 20130715 IS - 1399-0039 (Electronic) IS - 0001-2815 (Linking) VI - 82 IP - 2 DP - 2013 Aug TI - Identification of three novel human leukocyte antigen alleles, HLA-B*58:43, HLA-C*03:190, and HLA-DPA1*01:12, in an East African cohort. PG - 131-3 LID - 10.1111/tan.12157 [doi] AB - Three novel human leukocyte antigen (HLA) alleles were identified using a sequence-based typing of HLA class I and class II alleles of 1867 participants from a male circumcision cohort in Kenya. The new alleles were first identified by sequencing and then confirmed by cloning the polymerase chain reaction (PCR) products and sequencing multiple clones. HLA-B*58:43 was identical to HLA-B*58:02 with the exception of a nucleotide change at codon 125 in exon 3 (GCC-->ACC), and resulted in the amino acid change from Alanine to Threonine. HLA-C*03:190 was identical to HLA-C*03:02:01 with the exception of a nucleotide change at codon 131 in exon 3 (CGC-->TGC), and resulted in the amino acid change from Arginine to Cysteine. HLA-DPA1*01:12 was identical to HLA-DPA1*01:03:01:01 with the exception of a nucleotide change at codon 66 in exon 2 (TTG-->TCG), and resulted in the amino acid change from Leucine to Serine. CI - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Nykoluk, M AU - Nykoluk M AD - HIV and Human Genetics, National Microbiology Laboratory, Winnipeg, Canada. FAU - Bailey, R C AU - Bailey RC FAU - Moses, S AU - Moses S FAU - Plummer, F A AU - Plummer FA FAU - Luo, M AU - Luo M LA - eng SI - GENBANK/KC503840 SI - GENBANK/KC503841 SI - GENBANK/KC525951 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Tissue Antigens JT - Tissue antigens JID - 0331072 RN - 0 (Codon) RN - 0 (HLA-B Antigens) RN - 0 (HLA-C Antigens) RN - 0 (HLA-DP alpha-Chains) RN - 0 (HLA-DPA1 antigen) SB - IM MH - Africa, Eastern MH - *Alleles MH - Amino Acid Substitution MH - Base Sequence MH - Circumcision, Male MH - Codon MH - Exons MH - HLA-B Antigens/*genetics/immunology MH - HLA-C Antigens/*genetics/immunology MH - HLA-DP alpha-Chains/*genetics/immunology MH - Histocompatibility Testing MH - Humans MH - Male MH - Molecular Sequence Data MH - Sequence Alignment MH - Sequence Analysis, DNA OTO - NOTNLM OT - B*58:43 OT - C*03:190 OT - DPA1*01:12 OT - HLA-DPA1 OT - Kenyan OT - human leukocyte antigen-B OT - human leukocyte antigen-C OT - taxonomy-based sequence analysis EDAT- 2013/07/16 06:00 MHDA- 2014/01/28 06:00 CRDT- 2013/07/16 06:00 PHST- 2013/03/28 00:00 [received] PHST- 2013/05/06 00:00 [revised] PHST- 2013/05/28 00:00 [accepted] PHST- 2013/07/16 06:00 [entrez] PHST- 2013/07/16 06:00 [pubmed] PHST- 2014/01/28 06:00 [medline] AID - 10.1111/tan.12157 [doi] PST - ppublish SO - Tissue Antigens. 2013 Aug;82(2):131-3. doi: 10.1111/tan.12157.