PMID- 23850047
OWN - NLM
STAT- MEDLINE
DCOM- 20140516
LR  - 20131009
IS  - 1532-2653 (Electronic)
IS  - 0967-5868 (Linking)
VI  - 20
IP  - 10
DP  - 2013 Oct
TI  - An immunohistochemical study of increased tumor necrosis factor-alpha in the skin of 
      patients with amyotrophic lateral sclerosis.
PG  - 1371-6
LID - S0967-5868(13)00073-8 [pii]
LID - 10.1016/j.jocn.2012.11.007 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a major inflammatory cytokine that elicits a 
      wide range of biological responses and is implicated in the pathogenesis of 
      neurodegenerative diseases. Skin studies from patients with amyotrophic lateral 
      sclerosis (ALS) have shown unique pathological and biochemical abnormalities. The 
      lack of bedsore formation is considered characteristic of ALS. We undertook a 
      quantitative immunohistochemical study of TNF-alpha in the skin from patients with 
      ALS and controls with other neurologic or muscular diseases. Immunohistochemistry 
      for TNF-alpha demonstrated cytoplasmic activity in the epidermis and in some blood 
      vessels and glands. The proportion of TNF-alpha-positive (TNF-alpha+) cells in the 
      epidermis in patients with ALS was significantly higher (p<0.001) than in 
      controls. There was a significant positive relationship (r=0.87, p<0.001) between 
      this proportion and duration of illness in patients with ALS, but there was no 
      such relationship in control subjects. The optical density of TNF-alpha+ cells in the 
      epidermis in patients with ALS was markedly higher (p<0.001) than in controls. 
      There was a significant positive relationship (r=0.70, p<0.001) between the 
      immunoreactivity and duration of illness in patients with ALS. However, there was 
      no such relationship in controls. In addition, there was an appreciable positive 
      correlation (r=0.59, p<0.01) in patients with ALS between the proportion of 
      TNF-alpha+ cells and the optical density of these cells, but with no correlation in 
      controls. These data suggest that changes in TNF-alpha identified in the skin of 
      patients with ALS are likely to be related to the disease process and that 
      metabolic alterations of TNF-alpha may take place in the skin of patients with ALS.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Fukazawa, Hiroyuki
AU  - Fukazawa H
AD  - Department of Neurology, Teikyo University Chiba Medical Center, 3426-3 Anesaki, 
      Ichihara, Chiba 299-0111, Japan.
FAU - Tsukie, Tomomi
AU  - Tsukie T
FAU - Higashida, Kazuhiro
AU  - Higashida K
FAU - Fujikura, Mikio
AU  - Fujikura M
FAU - Ono, Seiitsu
AU  - Ono S
LA  - eng
PT  - Journal Article
DEP - 20130710
PL  - Scotland
TA  - J Clin Neurosci
JT  - Journal of clinical neuroscience : official journal of the Neurosurgical Society 
      of Australasia
JID - 9433352
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amyotrophic Lateral Sclerosis/*pathology/physiopathology
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Skin/*metabolism
MH  - Statistics as Topic
MH  - Tumor Necrosis Factor-alpha/*metabolism
OTO - NOTNLM
OT  - Amyotrophic lateral sclerosis
OT  - Cytokine
OT  - Epidermis
OT  - Immunohistochemistry
OT  - Skin
OT  - Tumor necrosis factor-alpha
EDAT- 2013/07/16 06:00
MHDA- 2014/05/17 06:00
CRDT- 2013/07/16 06:00
PHST- 2012/08/07 00:00 [received]
PHST- 2012/11/11 00:00 [revised]
PHST- 2012/11/13 00:00 [accepted]
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2014/05/17 06:00 [medline]
AID - S0967-5868(13)00073-8 [pii]
AID - 10.1016/j.jocn.2012.11.007 [doi]
PST - ppublish
SO  - J Clin Neurosci. 2013 Oct;20(10):1371-6. doi: 10.1016/j.jocn.2012.11.007. Epub 
      2013 Jul 10.