PMID- 23850781
OWN - NLM
STAT- MEDLINE
DCOM- 20140326
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 50
DP  - 2013 Dec 15
TI  - Mast cell-based electrochemical biosensor for quantification of the major shrimp 
      allergen Pen a 1 (tropomyosin).
PG  - 150-6
LID - S0956-5663(13)00429-6 [pii]
LID - 10.1016/j.bios.2013.06.032 [doi]
AB  - A novel cell-based electrochemical biosensor was developed to quantify major 
      shrimp allergen Pen a 1 (tropomyosin) and to assess its immunoglobulin E 
      (IgE)-mediated hypersensitivity. Rat basophilic leukemia (RBL-2H3) mast cells, 
      encapsulated in type I collagen, were immobilized on a self-assembled 
      l-cysteine/gold nanoparticle (AuNPsCys)-modified gold electrode to monitor 
      IgE-mediated mast cell sensitization and activation. The exposure of 
      dinitrophenol-bovine serum albumin (DNP-BSA), as a model antigen that stimulates 
      mast cells, induced a robust and long-lasting electrochemical impedance signal in 
      a dose-dependent manner which efficiently measured degranulation of anti-DNP 
      IgE-stimulated mast cells. Then this mast cell-based biosensor was applied into 
      quantification for the shrimp allergen with anti-shrimp tropomyosin 
      IgE-sensitization. The electrochemical impedance spectroscopy (EIS) results 
      showed that the impedance value (Ret) increased with the concentration of 
      purified shrimp allergen Pen a 1 (tropomyosin) in range of 0.5-0.25 mug mL(-1) 
      with the detection limit as 0.15 mug mL(-1), and the electrochemical result was 
      confirmed by beta-hexosaminidase assay and scanning electron microscopic 
      morphological (SEM) analysis. Thus, a simple, label-free, and sensitive method 
      for the determination of shrimp allergens was proposed and demonstrated here, 
      implying a highly versatile biosensor for food allergen detection and prediction.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Jiang, Donglei
AU  - Jiang D
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, Wuxi, Jiangsu 214122, China.
FAU - Ji, Jian
AU  - Ji J
FAU - An, Lu
AU  - An L
FAU - Sun, Xiulan
AU  - Sun X
FAU - Zhang, Yinzhi
AU  - Zhang Y
FAU - Zhang, Genyi
AU  - Zhang G
FAU - Tang, Lili
AU  - Tang L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130624
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Allergens)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Tropomyosin)
RN  - 0 (shrimp allergens Sa)
SB  - IM
MH  - Allergens/*analysis/immunology
MH  - Animals
MH  - Arthropod Proteins/*analysis/immunology
MH  - Biosensing Techniques/*instrumentation
MH  - Cell Line
MH  - Electrochemical Techniques/*instrumentation
MH  - Equipment Design
MH  - Food Analysis/*instrumentation
MH  - Limit of Detection
MH  - Mast Cells/drug effects/immunology
MH  - Penaeidae/*chemistry/immunology
MH  - Rats
MH  - Tropomyosin/*analysis
OTO - NOTNLM
OT  - Collagen
OT  - Electrochemical sensor
OT  - RBL-2H3 mast cells
OT  - Shrimp tropomyosin
EDAT- 2013/07/16 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/07/16 06:00
PHST- 2013/04/27 00:00 [received]
PHST- 2013/06/14 00:00 [revised]
PHST- 2013/06/14 00:00 [accepted]
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - S0956-5663(13)00429-6 [pii]
AID - 10.1016/j.bios.2013.06.032 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Dec 15;50:150-6. doi: 10.1016/j.bios.2013.06.032. Epub 
      2013 Jun 24.