PMID- 23851302
OWN - NLM
STAT- MEDLINE
DCOM- 20131217
LR  - 20131021
IS  - 1873-2399 (Electronic)
IS  - 0301-472X (Linking)
VI  - 41
IP  - 10
DP  - 2013 Oct
TI  - Genotypic and functional diversity of phenotypically defined primitive 
      hematopoietic cells in patients with chronic myeloid leukemia.
PG  - 837-47
LID - S0301-472X(13)00628-0 [pii]
LID - 10.1016/j.exphem.2013.07.001 [doi]
AB  - Much progress has been made in the management of chronic-phase chronic myeloid 
      leukemia (CP-CML), but there is a continuing imperative to develop curative 
      treatments, predict patient responses to specific modalities, and anticipate 
      disease relapse or progression. These needs underlie continuing interest in 
      methods to detect and quantify the relevant leukemic cells in clinical samples 
      with improved reliability and specificity. We report the results of comparing 
      three methods to enumerate primitive CP-CML cells in the same samples: genotyping 
      CD34(+)38(-) cells directly by fluorescence in situ hybridization, and measuring 
      BCR-ABL1 transcript-genotyped colony-forming cell outputs in either 5-week 
      long-term cultures (LTCs) containing non-engineered mouse fibroblasts or in 
      6-week LTCs containing mouse fibroblasts engineered to produce human Steel 
      factor, granulocyte colony-stimulating factor, and IL-3. The results demonstrate 
      that the first two methods significantly overestimate the prevalence of primitive 
      CP-CML cells by comparison to the third. In additional studies, we found that 
      CML-CD34(+) cells can repopulate the marrow and spleen of serially transplanted 
      adult NOD/SCID-IL-2Rgamma chain-null mice for more than 1 year with an almost 
      exclusive myeloid differentiation in primary and secondary recipients and without 
      evidence of disease progression. These findings underscore the importance of 
      long-term functional in vitro and in vivo endpoints to identify and characterize 
      CP-CML stem cells.
CI  - Crown Copyright (c) 2013. Published by Elsevier Inc. All rights reserved.
FAU - Sloma, Ivan
AU  - Sloma I
AD  - Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British 
      Columbia, Canada; Laboratoire d'Hematologie, Hopital Paul Brousse, Assistance 
      Publique des Hopitaux de Paris, Villejuif, France.
FAU - Beer, Philip A
AU  - Beer PA
FAU - Saw, Kyi Min
AU  - Saw KM
FAU - Chan, Matthew
AU  - Chan M
FAU - Leung, Donna
AU  - Leung D
FAU - Raghuram, Kamini
AU  - Raghuram K
FAU - Brimacombe, Cedric
AU  - Brimacombe C
FAU - Johnston, Bobby
AU  - Johnston B
FAU - Lambie, Karen
AU  - Lambie K
FAU - Forrest, Donna
AU  - Forrest D
FAU - Jiang, Xiaoyan
AU  - Jiang X
FAU - Eaves, Connie J
AU  - Eaves CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130711
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Genes, abl/genetics
MH  - *Genetic Variation
MH  - Genotype
MH  - Hematopoietic Stem Cells/cytology/metabolism/*pathology
MH  - Humans
MH  - In Situ Hybridization
MH  - Leukemia, Myeloid, Chronic-Phase/*genetics/*pathology
MH  - Male
MH  - Mice
MH  - Mice, SCID
MH  - Phenotype
MH  - Time Factors
EDAT- 2013/07/16 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/07/16 06:00
PHST- 2013/06/28 00:00 [received]
PHST- 2013/07/05 00:00 [accepted]
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - S0301-472X(13)00628-0 [pii]
AID - 10.1016/j.exphem.2013.07.001 [doi]
PST - ppublish
SO  - Exp Hematol. 2013 Oct;41(10):837-47. doi: 10.1016/j.exphem.2013.07.001. Epub 2013 
      Jul 11.