PMID- 23851653
OWN - NLM
STAT- MEDLINE
DCOM- 20140317
LR  - 20140113
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 159
IP  - 1
DP  - 2014 Jan
TI  - Dynamics and evolution of porcine reproductive and respiratory syndrome virus 
      (PRRSV) ORF5 following modified live PRRSV vaccination in a PRRSV-infected herd.
PG  - 17-27
LID - 10.1007/s00705-013-1781-9 [doi]
AB  - The objective of this study was to investigate the dynamics and evolution of 
      porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 following the 
      use of a modified live PRRSV (MLV) vaccine. A PRRSV-positive farm with 
      coexistence of types 1 and 2 and no history of MLV vaccination was investigated. 
      Vaccination with a type 2 MLV (Ingelvac PRRS MLV, Boehringer Ingelheim, USA) was 
      implemented. All sows were vaccinated at monthly intervals for two consecutive 
      months and then every third month. Piglets were vaccinated once at 7-10 days of 
      age and weaned to nursery facilities at 21-23 days of age. Serum samples were 
      collected monthly before and after vaccination from four population groups, 
      including replacement gilts and suckling, nursery and finishing pigs, and assayed 
      by PCR. After a year of blood collection, amplified products were sequenced, 
      resulting in 277 complete ORF5 gene sequences from 145 type 1 and 132 type 2 
      isolates. Prior to and following vaccination, both type 1 and type 2 PRRSV were 
      isolated and found to coexist in an individual pig. Each genotype evolved 
      separately without influencing the strain development of the other. Although the 
      substitution rates of both genotypes were relatively similar, MLV vaccination 
      appears to increase the heterogenicity of type 2 PRRSV, resulting in the 
      emergence of three novel type 2 PRRSV clusters in the herd, including an MLV-like 
      cluster, which disappeared within the month following whole-herd vaccination. Two 
      additional clusters included one related to the MLV vaccine and one related to 
      the endemic cluster of the herd.
FAU - Nilubol, Dachrit
AU  - Nilubol D
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Bangkok, 10330, Thailand, dachrit@gmail.com.
FAU - Tripipat, Thitima
AU  - Tripipat T
FAU - Hoonsuwan, Tawatchai
AU  - Hoonsuwan T
FAU - Tipsombatboon, Pavita
AU  - Tipsombatboon P
FAU - Piriyapongsa, Jittima
AU  - Piriyapongsa J
LA  - eng
SI  - GENBANK/JQ040720
SI  - GENBANK/JQ040721
SI  - GENBANK/JQ040722
SI  - GENBANK/JQ040723
SI  - GENBANK/JQ040724
SI  - GENBANK/JQ040725
SI  - GENBANK/JQ040726
SI  - GENBANK/JQ040727
SI  - GENBANK/JQ040728
SI  - GENBANK/JQ040729
SI  - GENBANK/JQ040730
SI  - GENBANK/JQ040731
SI  - GENBANK/JQ040732
SI  - GENBANK/JQ040733
SI  - GENBANK/JQ040734
SI  - GENBANK/JQ040735
SI  - GENBANK/JQ040736
SI  - GENBANK/JQ040737
SI  - GENBANK/JQ040738
SI  - GENBANK/JQ040739
SI  - GENBANK/JQ040740
SI  - GENBANK/JQ040741
SI  - GENBANK/JQ040742
SI  - GENBANK/JQ040743
SI  - GENBANK/JQ040744
SI  - GENBANK/JQ040745
SI  - GENBANK/JQ040746
SI  - GENBANK/JQ040747
SI  - GENBANK/JQ040748
SI  - GENBANK/JQ040749
SI  - GENBANK/JQ040750
SI  - GENBANK/JQ040751
SI  - GENBANK/JQ040752
SI  - GENBANK/JQ040753
SI  - GENBANK/JQ040754
SI  - GENBANK/JQ040755
SI  - GENBANK/JQ040756
SI  - GENBANK/JQ040757
SI  - GENBANK/JQ040758
SI  - GENBANK/JQ040759
SI  - GENBANK/JQ040760
SI  - GENBANK/JQ040761
SI  - GENBANK/JQ040762
SI  - GENBANK/JQ040763
SI  - GENBANK/JQ040764
SI  - GENBANK/JQ040765
SI  - GENBANK/JQ040766
SI  - GENBANK/JQ040767
SI  - GENBANK/JQ040768
SI  - GENBANK/JQ040769
SI  - GENBANK/JQ040770
SI  - GENBANK/JQ040771
SI  - GENBANK/JQ040772
SI  - GENBANK/JQ040773
SI  - GENBANK/JQ040774
SI  - GENBANK/JQ040775
SI  - GENBANK/JQ040776
SI  - GENBANK/JQ040777
SI  - GENBANK/JQ040778
SI  - GENBANK/JQ040779
SI  - GENBANK/JQ040780
SI  - GENBANK/JQ040781
SI  - GENBANK/JQ040782
SI  - GENBANK/JQ040783
SI  - GENBANK/JQ040784
SI  - GENBANK/JQ040785
SI  - GENBANK/JQ040786
SI  - GENBANK/JQ040787
SI  - GENBANK/JQ040788
SI  - GENBANK/JQ040789
SI  - GENBANK/JQ040790
SI  - GENBANK/JQ040791
SI  - GENBANK/JQ040792
SI  - GENBANK/JQ040793
SI  - GENBANK/JQ040794
SI  - GENBANK/JQ040795
SI  - GENBANK/JQ040796
SI  - GENBANK/JQ040797
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130713
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - *Evolution, Molecular
MH  - Female
MH  - Genotype
MH  - Male
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & 
      control/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/immunology/isolation & purification
MH  - Sequence Alignment
MH  - Swine
MH  - Vaccination
MH  - Viral Proteins/administration & dosage/chemistry/*genetics/immunology
MH  - Viral Vaccines/administration & dosage/chemistry/*genetics/immunology
EDAT- 2013/07/16 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/07/16 06:00
PHST- 2013/02/21 00:00 [received]
PHST- 2013/05/29 00:00 [accepted]
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 10.1007/s00705-013-1781-9 [doi]
PST - ppublish
SO  - Arch Virol. 2014 Jan;159(1):17-27. doi: 10.1007/s00705-013-1781-9. Epub 2013 Jul 
      13.