PMID- 23852505
OWN - NLM
STAT- MEDLINE
DCOM- 20140505
LR  - 20180310
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 789
DP  - 2013
TI  - Radiation affects the responsiveness of bone marrow to G-CSF.
PG  - 273-280
LID - 10.1007/978-1-4614-7411-1_37 [doi]
AB  - In this study, we investigated the response of irradiated bone marrow cells to 
      granulocyte colony-stimulating factor (G-CSF). Freshly harvested bone marrow 
      cells were treated with either saline (vehicle control) or 20 ng/ml of G-CSF. 
      Thereafter, cells were separated into nonirradiated (no-IR) and irradiated (IR, 
      0.5 Gy) groups. IR cells exhibited a higher proliferation rate in response to 
      G-CSF, as compared to the no-IR cells. Reduced levels of reactive oxygen species 
      indicated that G-CSF-treated IR cells produced fewer free radicals, as compared 
      to the no-IR cells. The G-CSF-treated IR cells also had a lower apoptotic rate 
      than their no-IR counterparts. Furthermore, G-CSF-treated IR cells exhibited less 
      alteration of mitochondrial membrane potential, as compared to the no-IR cells. 
      Finally, the mitochondrial number increased in the G-CSF-treated IR cells. The 
      radiation-induced increase in plasma IL-6 in vivo could be enhanced by the 
      administration of G-CSF. The data suggest that radiation potentiates the response 
      of bone marrow cells to G-CSF treatment.
FAU - Zhang, Zhenhuan
AU  - Zhang Z
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Zhang, Mei
AU  - Zhang M
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Lv, Wenlong
AU  - Lv W
AD  - Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical 
      University, Fuzhou, Fujian, 35001, China.
FAU - Huang, Luqiang
AU  - Huang L
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Yin, Liangjie
AU  - Yin L
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Yang, Shanmin
AU  - Yang S
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Hong, Jinsheng
AU  - Hong J
AD  - Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical 
      University, Fuzhou, Fujian, 35001, China.
FAU - Han, Deping
AU  - Han D
AD  - Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical 
      University, Fuzhou, Fujian, 35001, China.
FAU - Chen, Chun
AU  - Chen C
AD  - Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical 
      University, Fuzhou, Fujian, 35001, China.
FAU - Zhang, Amy
AU  - Zhang A
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Vidyasagar, Sadasivan
AU  - Vidyasagar S
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Okunieff, Paul
AU  - Okunieff P
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA.
FAU - Zhang, Lurong
AU  - Zhang L
AD  - Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 
      Gainesville, FL, 32610, USA. lurongzhang@ufl.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Free Radicals)
RN  - 0 (Interleukin-6)
RN  - 0 (Reactive Oxygen Species)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow/*drug effects/metabolism/*radiation effects
MH  - Bone Marrow Cells/drug effects/metabolism/radiation effects
MH  - Free Radicals/metabolism
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - Interleukin-6/blood
MH  - Male
MH  - Membrane Potential, Mitochondrial/drug effects/radiation effects
MH  - Mice
MH  - Mitochondria/drug effects/metabolism/radiation effects
MH  - Reactive Oxygen Species/metabolism
EDAT- 2013/07/16 06:00
MHDA- 2014/05/06 06:00
CRDT- 2013/07/16 06:00
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2014/05/06 06:00 [medline]
AID - 10.1007/978-1-4614-7411-1_37 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 2013;789:273-280. doi: 10.1007/978-1-4614-7411-1_37.