PMID- 23853757
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141118
LR  - 20211021
IS  - 2193-1801 (Print)
IS  - 2193-1801 (Electronic)
IS  - 2193-1801 (Linking)
VI  - 2
IP  - 1
DP  - 2013 Dec
TI  - Enhancing nitrilase production from Fusarium proliferatum using response surface 
      methodology.
PG  - 290
LID - 10.1186/2193-1801-2-290 [doi]
LID - 290
AB  - The individual and interactive effects of three independent variables i.e. carbon 
      source (glucose), nitrogen source (sodium nitrate) and inducer (ϵ-caprolactam) on 
      nitrilase production from Fusarium proliferatum were investigated using design of 
      experiments (DOE) methodology. Response surface methodology (RSM) was followed to 
      generate the process model and to obtain the optimal conditions for maximum 
      nitrilase production. Based on central composite design (CCD) a quadratic model 
      was found to fit the experimental data (p<0.0001) and maximum activity of 59.0U/g 
      biomass was predicted at glucose concentration (53.22 g/l), sodium nitrate 
      (2.31 g/l) and ϵ-caprolactam (3.58 g/l). Validation experiments were carried out 
      under the optimized conditions for verification of the model. The nitrilase 
      activity of 58.3U/g biomass obtained experimentally correlated to the predicted 
      activity which proves the authenticity of the model. Overall 2.24 fold increase 
      in nitrilase activity was achieved as compared to the activity before 
      optimization (26U/g biomass).
FAU - Yusuf, Farnaz
AU  - Yusuf F
AD  - Fermentation Technology Division, Indian Institute of Integrative Medicine, Canal 
      Road, Jammu Tawi, 180001 India.
FAU - Chaubey, Asha
AU  - Chaubey A
FAU - Raina, Arvind
AU  - Raina A
FAU - Jamwal, Urmila
AU  - Jamwal U
FAU - Parshad, Rajinder
AU  - Parshad R
LA  - eng
PT  - Journal Article
DEP - 20130701
PL  - Switzerland
TA  - Springerplus
JT  - SpringerPlus
JID - 101597967
PMC - PMC3706717
OTO - NOTNLM
OT  - Central composite design
OT  - Design of experiments
OT  - Fusarium proliferatum
OT  - Nitrilase
OT  - Response surface methodology
EDAT- 2013/07/16 06:00
MHDA- 2013/07/16 06:01
PMCR- 2013/07/01
CRDT- 2013/07/16 06:00
PHST- 2013/01/16 00:00 [received]
PHST- 2013/06/19 00:00 [accepted]
PHST- 2013/07/16 06:00 [entrez]
PHST- 2013/07/16 06:00 [pubmed]
PHST- 2013/07/16 06:01 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - 359 [pii]
AID - 10.1186/2193-1801-2-290 [doi]
PST - epublish
SO  - Springerplus. 2013 Jul 1;2(1):290. doi: 10.1186/2193-1801-2-290. Print 2013 Dec.