PMID- 2385616
OWN - NLM
STAT- MEDLINE
DCOM- 19900917
LR  - 20190912
IS  - 0952-3278 (Print)
IS  - 0952-3278 (Linking)
VI  - 40
IP  - 2
DP  - 1990 Jun
TI  - Transformation of arachidonic acid by 5- and 15-lipoxygenase pathways in bovine 
      adrenal fasciculata cells.
PG  - 93-102
AB  - [1-14C]Arachidonic acid was incubated with isolated bovine adrenal fasciculata 
      cells for 15 min at 37gC. The metabolites were separated and purified by reverse- 
      and straight-phase high performance liquid chromatography, and identified by gas 
      chromatography-mass spectrometry or radioimmunoassay. Identified metabolites were 
      5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), 
      15-hydroxy-5,8,11,13-eicosatetraenoic acid (15-HETE), leukotriene B4 and 
      11,14,15-trihydroxy-5,8,12-eicosatrienoic acid (11,14,15-THET). Addition of 
      15-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE), an intermediate 
      metabolite of 15-lipoxygenase pathway to microsomes of bovine adrenal fasciculata 
      cells resulted in the formation of 11,14,15-THET. The formation of 11,14,15-THET 
      by microsomes was not dependent on the presence of NADPH, while it was 
      dose-dependently suppressed by ketoconazole, a potent inhibitor of cytochrome 
      P-450 dependent enzymes. These results indicate that 5- and 15-lipoxygenase 
      pathways of arachidonic acid may exist in bovine adrenal fasciculata cells and 
      that 15-HPETE is further metabolized to 11,14,15-THET by adrenal microsomal 
      cytochrome P-450.
FAU - Omura, M
AU  - Omura M
AD  - Second Department of Internal Medicine, School of Medicine, Chiba University, 
      Japan.
FAU - Hirai, A
AU  - Hirai A
FAU - Tamura, Y
AU  - Tamura Y
FAU - Yoshida, S
AU  - Yoshida S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Scotland
TA  - Prostaglandins Leukot Essent Fatty Acids
JT  - Prostaglandins, leukotrienes, and essential fatty acids
JID - 8802730
RN  - 0 (Arachidonic Acids)
RN  - 0 (Cytochrome P-450 Enzyme Inhibitors)
RN  - EC 1.13.11.- (Arachidonate Lipoxygenases)
RN  - EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
RN  - R9400W927I (Ketoconazole)
SB  - IM
MH  - Animals
MH  - Arachidonate 15-Lipoxygenase/*metabolism
MH  - Arachidonate 5-Lipoxygenase/*metabolism
MH  - Arachidonate Lipoxygenases/*metabolism
MH  - Arachidonic Acids/*metabolism
MH  - Cattle
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Cytochrome P-450 Enzyme Inhibitors
MH  - In Vitro Techniques
MH  - Ketoconazole/pharmacology
MH  - Male
MH  - Microsomes/drug effects/enzymology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Zona Fasciculata/cytology/drug effects/*enzymology
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
AID - 10.1016/0952-3278(90)90150-j [doi]
PST - ppublish
SO  - Prostaglandins Leukot Essent Fatty Acids. 1990 Jun;40(2):93-102. doi: 
      10.1016/0952-3278(90)90150-j.