PMID- 23859311
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20140116
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 90
IP  - 1
DP  - 2014 Jan
TI  - Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced 
      by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. 
      pendulum--Solanaceae).
PG  - 104-12
LID - 10.3109/09553002.2013.825064 [doi]
AB  - PURPOSE: To locate transient chromosome aberrations on a selected pepper cultivar 
      and determine the tracing efficiency of different cytogenetic methods. MATERIALS 
      AND METHODS: Seeds from Capsicum baccatum var. pendulum cultivar 'Cayenne' were 
      treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were 
      analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus 
      organizer regions (silver positive nucleolus organizing regions or AgNOR), 
      fluorescent banding, fluorescence in situ hybridization (FISH) and meiotic 
      analysis]. RESULTS: A rearranged chromosome carrying two nucleolus organizing 
      regions (NOR) induced by ionizing radiation was detected in the cultivar, with 
      the occurrence of a small reciprocal exchange between a chromosome of pair no. 1 
      and another chromosome of pair no. 3, both carrying active NOR in short arms and 
      associated chromomycin A positive/diamidino-phenylindole negative (CMA+/DAPI-) 
      heterochromatin. Meiotic analysis showed a quadrivalent configuration, confirming 
      a reciprocal translocation between two chromosomes. CONCLUSIONS: The use of 
      X-rays in Capsicum allowed us to develop and identify a pepper line with 
      structural rearrangements between two NOR-carrying chromosomes. We postulate that 
      all the cytological techniques employed in this research were efficient in the 
      search for chromosome aberrations. Particularly, Feulgen and AgNOR were the most 
      suitable in those cases of transient rearrangements, whereas fluorescent banding 
      and FISH were appropriate for intransitive ones.
FAU - Scaldaferro, Marisel A
AU  - Scaldaferro MA
AD  - Instituto Multidisciplinario de Biologia Vegetal (IMBIV-CONICET) , Cordoba.
FAU - Grabiele, Mauro
AU  - Grabiele M
FAU - Seijo, J Guillermo
AU  - Seijo JG
FAU - Debat, Humberto
AU  - Debat H
FAU - Romero, M Victoria
AU  - Romero MV
FAU - Ducasse, Daniel A
AU  - Ducasse DA
FAU - Prina, Alberto R
AU  - Prina AR
FAU - Moscone, Eduardo A
AU  - Moscone EA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130809
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Base Sequence
MH  - Capsicum/*genetics/*radiation effects
MH  - Chromosome Aberrations/*radiation effects
MH  - Cytogenetic Analysis/*methods
MH  - DNA, Plant/*genetics/*radiation effects
MH  - Gene Rearrangement/*genetics/radiation effects
MH  - Molecular Sequence Data
MH  - Radiation Dosage
EDAT- 2013/07/19 06:00
MHDA- 2014/03/07 06:00
CRDT- 2013/07/18 06:00
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - 10.3109/09553002.2013.825064 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2014 Jan;90(1):104-12. doi: 10.3109/09553002.2013.825064. Epub 
      2013 Aug 9.