PMID- 23860397
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20240413
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 19
DP  - 2013 Jul 17
TI  - Association between infection of virulence cagA gene Helicobacter pylori and 
      laryngeal squamous cell carcinoma.
PG  - 584-91
LID - 10.12659/MSM.889011 [doi]
AB  - BACKGROUND: The aim of the study was to evaluate the presence of cagA gene 
      Helicobacter pylori in etiopathogenesis of initiation and development of larynx 
      squamous cell carcinoma (LSCC) and its predictable role as a prognostic factor. 
      MATERIAL AND METHODS: The prospective, controlled study involved a series of 75 
      patients (65 male, 10 female, mean age 59.1 years, range 43 to 79 years) with 
      larynx cancer. Samples of larynx cancerous tissue, each of 10-15 mg, were 
      obtained from fresh tissues and were used for nucleic acid purification. DNA was 
      extracted from 225 samples (larynx tumor - I (75), margin of tumor and normal 
      tissue - II (75) and normal larynx tissue from opposite side to the tumor - III). 
      All samples were subjected to H. pylori ureA detection by the PCR H. pylori 
      diagnostic test. Samples that were positive for ureA H. pylori gene were 
      evaluated for cagA H. pylori gene. RESULTS: Presence of H. pylori cagA gene was 
      identified in 46,7% to 49,3% of 75 H. pylori ureA gene-positive larynx cancer 
      depending of tissue location. There was a correlation of high incidence of 
      positive cagA gene in larynx cancer tissue in supraglottic versus subglottic and 
      glottic location. We observed a predominance of cagA gene in LSCC in patients 
      with positive cervical lymph nodes and clinical stage T3 and T4. CONCLUSIONS: H. 
      pylori is present in larynx tissue and may be a possible carcinogen or 
      co-carcinogen in LSCC development, but that must be addressed by future 
      investigations. The presence of cagA gene in larynx cancer tissues significantly 
      decreases survival rate and increases the disease recurrence possibilities.
FAU - Burduk, Pawel Krzysztof
AU  - Burduk PK
AD  - Nicolaus Copernicus University of Torun, Bydgoszcz, Poland. pburduk@wp.pl
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130717
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (cagA protein, Helicobacter pylori)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Bacterial/*genetics
MH  - Bacterial Proteins/*genetics
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*complications/microbiology/mortality/pathology
MH  - Female
MH  - Genes, Bacterial/genetics
MH  - Helicobacter Infections/*complications/*microbiology/mortality/pathology
MH  - Helicobacter pylori/genetics/*pathogenicity
MH  - Humans
MH  - Laryngeal Neoplasms/*complications/*microbiology/mortality/pathology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Poland/epidemiology
MH  - Polymerase Chain Reaction
MH  - Recurrence
MH  - Sequence Analysis, DNA
MH  - Survival Rate
MH  - Virulence/genetics
PMC - PMC3718723
EDAT- 2013/07/19 06:00
MHDA- 2013/10/22 06:00
PMCR- 2013/07/17
CRDT- 2013/07/18 06:00
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
PHST- 2013/07/17 00:00 [pmc-release]
AID - 889011 [pii]
AID - 10.12659/MSM.889011 [doi]
PST - epublish
SO  - Med Sci Monit. 2013 Jul 17;19:584-91. doi: 10.12659/MSM.889011.