PMID- 23860759
OWN - NLM
STAT- MEDLINE
DCOM- 20130930
LR  - 20151119
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 54
IP  - 8
DP  - 2013 Aug 7
TI  - Wide-field laser ophthalmoscopy for mice: a novel evaluation system for 
      retinal/choroidal angiogenesis in mice.
PG  - 5288-93
LID - 10.1167/iovs.13-11946 [doi]
AB  - PURPOSE: The purpose of this study was to investigate the application of 
      wide-field laser ophthalmoscopy (Optos) for the evaluation of established models 
      of angiogenesis and the healthy retina in mice. METHODS: To investigate whether 
      angiogenesis and leakage in the retina and choroid can be evaluated with Optos, 
      we used two models of angiogenesis: oxygen-induced retinopathy (OIR) and 
      laser-induced choroidal neovascularization (CNV). Fundus imaging and fluorescein 
      angiography (FAG) were performed with the Optos system without a contact lens. 
      Furthermore, to evaluate in vivo leukocyte infiltration in these models, we 
      injected acridine orange (AO) and performed imaging using Optos. RESULTS: In vivo 
      fundus imaging with Optos did not require any additional optical device. 
      Additionally, Optos enabled us to repeatedly obtain high-resolution color images 
      and FAG images in the OIR model as well as in the CNV model in mice. Through a 
      combination of Optos imaging and AO fluorography, the number and location of the 
      infiltrating leukocytes could be identified in these models. CONCLUSIONS: Optos 
      is a wide-viewing imaging tool for the noninvasive in vivo evaluation of common 
      angiogenesis models, oxygen-induced retinopathy and laser-induced choroidal 
      neovascularization, as well as the healthy retina in mice.
FAU - Nakao, Shintaro
AU  - Nakao S
AD  - Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan. snakao@med.kyushu-u.ac.jp
FAU - Arita, Ryoichi
AU  - Arita R
FAU - Nakama, Takahito
AU  - Nakama T
FAU - Yoshikawa, Hiroshi
AU  - Yoshikawa H
FAU - Yoshida, Shigeo
AU  - Yoshida S
FAU - Enaida, Hiroshi
AU  - Enaida H
FAU - Hafezi-Moghadam, Ali
AU  - Hafezi-Moghadam A
FAU - Matsui, Takaaki
AU  - Matsui T
FAU - Ishibashi, Tatsuro
AU  - Ishibashi T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130807
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Choroidal Neovascularization/chemically induced/*diagnosis
MH  - Disease Models, Animal
MH  - Equipment Design
MH  - Fluorescein Angiography
MH  - Fluorescent Dyes
MH  - Fundus Oculi
MH  - *Lasers
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Ophthalmoscopes
MH  - Ophthalmoscopy/*methods
MH  - Oxygen/toxicity
MH  - Reproducibility of Results
MH  - Retinal Neovascularization/chemically induced/*diagnosis
OTO - NOTNLM
OT  - Optos
OT  - choroidal neovascularization
OT  - imaging
OT  - leakage
OT  - oxygen-induced retinopathy
OT  - quantitation
EDAT- 2013/07/19 06:00
MHDA- 2013/10/01 06:00
CRDT- 2013/07/18 06:00
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2013/10/01 06:00 [medline]
AID - iovs.13-11946 [pii]
AID - 10.1167/iovs.13-11946 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2013 Aug 7;54(8):5288-93. doi: 10.1167/iovs.13-11946.