PMID- 23860800
OWN - NLM
STAT- MEDLINE
DCOM- 20151217
LR  - 20211021
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 21
IP  - 3
DP  - 2015 Mar
TI  - Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and 
      tissue engineered cartilage in vivo.
PG  - 196-203
LID - 10.1007/s11655-013-1511-x [doi]
AB  - OBJECTIVE: Although chondroprotective activities have been documented for 
      polysaccharides, the potential target of different polysaccharide may differ. The 
      study was aimed to explore the effect of glucan HBP-A in chondrocyte monolayer 
      culture and chondrocytes-alginate hydrogel constructs in vivo, especially on the 
      expression of type II collagen. METHODS: Chondrocytes isolated from rabbit 
      articular cartilage were cultured and verified by immunocytochemical staining of 
      type II collagen. Chondrocyte viability was assessed after being treated with 
      HBP-A in different concentrations. Morphological status of chondrocytes-alginate 
      hydrogel constructs in vitro was observed by scanning electron microscope (SEM). 
      The constructs were treated with HBP-A and then injected to nude mice 
      subcutaneously. Six weeks after transplantation, the specimens were observed 
      through transmission electron microscopy (TEM). The mRNA expressions of 
      disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTs-5), 
      aggrecan and type II collagen in both monolayer culture and constructs were 
      determined by real time polymerase chain reaction (PCR). The expression of type 
      II collagen and matrix metalloproteinases-3 (MMP-3) in chondrocyte monolayer 
      culture was also tested through Western blot and enzyme linked immunosorbent 
      assay (ELISA), respectively. RESULTS: MMP-3 secretion and ADAMTs-5 mRNA 
      expression in vitro were inhibited by HBP-A at 0.3 mg/mL concentration. In 
      morphological study, there were significant appearance of collagen in those 
      constructs treated by HBP-A. Accordingly, in both chondrocyte monolayer culture 
      and chondrocytes-alginate hydrogel constructs, the expression of type II collagen 
      was increased significantly in HBP-A group when compared with control group 
      (P<0.001). CONCLUSIONS: The study documented that the potential pharmacological 
      target of glucan HBP-A in chondrocytes monolayer culture and tissue engineered 
      cartilage in vivo may be concerned with the inhibition of catabolic enzymes 
      MMP-3, ADAMTs-5, and increasing of type II collagen expression.
FAU - Cao, Yue-long
AU  - Cao YL
AD  - Research Institute of Orthopaedics, Shuguang Hospital Affiliated to Shanghai 
      University of Traditional Chinese Medicine, Shanghai, 201203, China, 
      ningtcm@126.com.
FAU - Liu, Ting
AU  - Liu T
FAU - Pang, Jian
AU  - Pang J
FAU - Gao, Ning-yang
AU  - Gao NY
FAU - Zhan, Hong-sheng
AU  - Zhan HS
FAU - Shi, Yin-yu
AU  - Shi YY
FAU - Wang, Xiang
AU  - Wang X
FAU - Wang, Shun-chun
AU  - Wang SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130716
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Aggrecans)
RN  - 0 (Alginates)
RN  - 0 (Collagen Type II)
RN  - 0 (Glucans)
RN  - 0 (Hexuronic Acids)
RN  - 0 (RNA, Messenger)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - ADAM Proteins/genetics/metabolism
MH  - Aggrecans/genetics/metabolism
MH  - Alginates/pharmacology
MH  - Animals
MH  - Cartilage, Articular/drug effects/*physiology
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Chondrocytes/cytology/drug effects/*metabolism/ultrastructure
MH  - Collagen Type II/genetics/*metabolism
MH  - Female
MH  - Glucans/*pharmacology
MH  - Glucuronic Acid/pharmacology
MH  - Hexuronic Acids/pharmacology
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/pharmacology
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Mice, Nude
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Tissue Engineering/*methods
EDAT- 2013/07/19 06:00
MHDA- 2015/12/19 06:00
CRDT- 2013/07/18 06:00
PHST- 2011/05/10 00:00 [received]
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2015/12/19 06:00 [medline]
AID - 10.1007/s11655-013-1511-x [doi]
PST - ppublish
SO  - Chin J Integr Med. 2015 Mar;21(3):196-203. doi: 10.1007/s11655-013-1511-x. Epub 
      2013 Jul 16.