PMID- 23861876
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 7
DP  - 2013
TI  - Single cell gene profiling revealed heterogeneity of paracrine effects of bone 
      marrow cells in mouse infarcted hearts.
PG  - e68270
LID - 10.1371/journal.pone.0068270 [doi]
LID - e68270
AB  - It is now recognized that transplantation of bone marrow cells (BMCs) into 
      infarcted hearts has the capacity to improve the cardiac function through 
      paracrine effects. However, detailed expression levels of paracrine factors in 
      BMCs in infarcted hearts are poorly described. By use of laser capture 
      microdissection combined with real-time PCR, we depicted the expression profiles 
      of paracrine factors in infarcted hearts versus normal hearts. Consistent with 
      the in vivo observation, a similar expression pattern was evidenced in cultured 
      BMCs. Furthermore, BMCs displayed heterogeneity of paracrine effects in infarcted 
      hearts as analyzed at the single cell level using single cell PCR. Interestingly, 
      the CD45(+) subpopulation showed higher expression levels of angiogenic factors 
      compared to other subpopulations. Finally, most angiogenic factors were induced 
      under the microenvironment of infarction. Our study demonstrated the 
      heterogeneity of paracrine effects in BMCs at single cell level in infarcted 
      hearts, highlighting preferential expression of angiogenic factors in the CD45(+) 
      subpopulation. These findings broaden our understanding of paracrine effects of 
      BMCs in vivo, and offer new insights into BMCs therapy in myocardial infarction 
      (MI).
FAU - Li, Yanhua
AU  - Li Y
AD  - Institute of Geriatric Cardiology, the General Hospital of Chinese People's 
      Liberation Army (PLA), Beijing, China.
FAU - Guo, Xinhong
AU  - Guo X
FAU - Xue, Qiao
AU  - Xue Q
FAU - Zhu, Mei
AU  - Zhu M
FAU - Gao, Lei
AU  - Gao L
FAU - Wang, Yu
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130705
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Angiogenesis Inducing Agents)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Angiogenesis Inducing Agents/metabolism
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - Bone Marrow Transplantation
MH  - Cluster Analysis
MH  - Disease Models, Animal
MH  - Female
MH  - Heart/physiopathology
MH  - Hypoxia
MH  - Leukocyte Common Antigens/metabolism
MH  - Mice
MH  - Myocardial Infarction/*genetics/*metabolism
MH  - Myocardium/metabolism/pathology
MH  - Neovascularization, Pathologic
MH  - *Paracrine Communication
MH  - Single-Cell Analysis
MH  - *Transcriptome
PMC - PMC3702556
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/07/19 06:00
MHDA- 2014/02/14 06:00
PMCR- 2013/07/05
CRDT- 2013/07/18 06:00
PHST- 2013/01/01 00:00 [received]
PHST- 2013/05/28 00:00 [accepted]
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
PHST- 2013/07/05 00:00 [pmc-release]
AID - PONE-D-13-00920 [pii]
AID - 10.1371/journal.pone.0068270 [doi]
PST - epublish
SO  - PLoS One. 2013 Jul 5;8(7):e68270. doi: 10.1371/journal.pone.0068270. Print 2013.