PMID- 23862157
OWN - NLM
STAT- MEDLINE
DCOM- 20140204
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2013
DP  - 2013
TI  - Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kutzing 
      extract in experimental type 2 diabetic rats.
PG  - 820786
LID - 10.1155/2013/820786 [doi]
LID - 820786
AB  - Spirogyra neglecta extract (SNE) has shown antihyperglycemia and 
      antihyperlipidemia in type 2 diabetic mellitus (T2DM) rats. This study 
      investigated the antioxidant and renoprotective effects of SNE in T2DM rats 
      induced by high-fat diet with low-single dose streptozotocin. T2DM rats were fed 
      daily with SNE (0.25, 0.5, and 1 g/kg BW) for 12 weeks. Renal morphology, 
      malondialdehyde levels, qPCR, and western blotting were analyzed. Renal cortical 
      slices were used to determine renal transport of organic anions, which are 
      estrone sulfate and para-aminohippurate, mediated through organic anion 
      transporter 3-Oat3. Insulin and PKCzeta were known to activate Oat3 function while 
      it was inhibited by PKCalpha. Compared to T2DM, plasma glucose, triglyceride, insulin 
      resistance, renal morphology, and malondialdehyde levels were significantly 
      improved by SNE supplementation. Reduced glutathione peroxidase and nuclear 
      factor kappaB expressions were related to antioxidant effect of SNE. Oat3 mRNA and 
      protein were not different among groups, but insulin-stimulated rOat3 followed by 
      anion uptakes was abolished in T2DM. This was restored in the slices from SNE 
      treatment. The mechanism of SNE-improved Oat3 was associated with PKCalpha and PKCzeta 
      expressions and activities. These findings indicate that SNE has beneficial 
      effects on renal transport through antioxidant enzymes and PKCs in T2DM rats.
FAU - Ontawong, Atcharaporn
AU  - Ontawong A
AD  - Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 
      50200, Thailand.
FAU - Saowakon, Naruwan
AU  - Saowakon N
FAU - Vivithanaporn, Pornpun
AU  - Vivithanaporn P
FAU - Pongchaidecha, Anchalee
AU  - Pongchaidecha A
FAU - Lailerd, Narissara
AU  - Lailerd N
FAU - Amornlerdpison, Doungporn
AU  - Amornlerdpison D
FAU - Lungkaphin, Anusorn
AU  - Lungkaphin A
FAU - Srimaroeng, Chutima
AU  - Srimaroeng C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130603
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Antioxidants)
RN  - 0 (Insulin)
RN  - 0 (Organic Anion Transporters, Sodium-Independent)
RN  - 0 (Plant Extracts)
RN  - 0 (Protective Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (organic anion transport protein 3)
RN  - 2DI9HA706A (Estrone)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 2.7.- (Protein Kinases)
RN  - QTL48N278K (estrone sulfate)
RN  - Y79XT83BJ9 (p-Aminohippuric Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology/*therapeutic use
MH  - Biological Transport/drug effects
MH  - Diabetes Mellitus, Experimental/*drug therapy
MH  - Diabetes Mellitus, Type 2/*drug therapy/pathology
MH  - Estrone/analogs & derivatives/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Insulin/pharmacology
MH  - Kidney/drug effects/*pathology
MH  - Kidney Cortex/drug effects/pathology
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Organic Anion Transporters, Sodium-Independent/genetics/metabolism
MH  - Phytotherapy
MH  - Plant Extracts/pharmacology/*therapeutic use
MH  - Protective Agents/pharmacology/*therapeutic use
MH  - Protein Kinases/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Spirogyra/*chemistry
MH  - Staining and Labeling
MH  - Stress, Physiological/drug effects
MH  - p-Aminohippuric Acid/metabolism
PMC - PMC3686068
EDAT- 2013/07/19 06:00
MHDA- 2014/02/05 06:00
PMCR- 2013/06/03
CRDT- 2013/07/18 06:00
PHST- 2013/02/20 00:00 [received]
PHST- 2013/04/24 00:00 [revised]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2014/02/05 06:00 [medline]
PHST- 2013/06/03 00:00 [pmc-release]
AID - 10.1155/2013/820786 [doi]
PST - ppublish
SO  - Biomed Res Int. 2013;2013:820786. doi: 10.1155/2013/820786. Epub 2013 Jun 3.