PMID- 23864501
OWN - NLM
STAT- MEDLINE
DCOM- 20140204
LR  - 20211021
IS  - 1742-5662 (Electronic)
IS  - 1742-5689 (Print)
IS  - 1742-5662 (Linking)
VI  - 10
IP  - 86
DP  - 2013 Sep 6
TI  - Applicability of ToF-SIMS for monitoring compositional changes in bone in a 
      long-term animal model.
PG  - 20130332
LID - 10.1098/rsif.2013.0332 [doi]
LID - 20130332
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a well-established 
      technique in material sciences but has not yet been widely explored for 
      implementation in life sciences. Here, we demonstrate the applicability and 
      advantages of ToF-SIMS analysis for the study of minerals and biomolecules in 
      osseous tissue. The locally resolved analysis of fragment ions deriving from the 
      sample surface enables imaging and differentiation of bone tissue and facilitates 
      histology on non-stained cross sections. In a rat model, bilateral ovariectomy 
      combined with either a multi-deficiency diet or steroid treatment was carried out 
      to create osteoporotic conditions. We focused our study on the Ca content of the 
      mineralized tissue and monitored its decline. Calcium mass images of cross 
      sections show the progressive degenerative changes in the bone. We observed a 
      decreased Ca concentration in the edge region of the trabeculae and a decline in 
      the Ca/P ratio. Additionally, we focused on the non-mineralized matrix and 
      identified fragment ions that are characteristic for the collagen matrix. We 
      observed trabeculae with wide ranges of non-mineralized collagen for the diet 
      group owing to an impaired mineralization process. Here, the advantage of coeval 
      monitoring of collagen and minerals indicated an osteomalacic model rather than 
      an osteoporotic one.
FAU - Henss, Anja
AU  - Henss A
AD  - Institute for Physical Chemistry, Justus-Liebig-University of Giessen, 
      Heinrich-Buff-Ring 58, 35392 Giessen, Germany.
FAU - Rohnke, Marcus
AU  - Rohnke M
FAU - El Khassawna, Thaqif
AU  - El Khassawna T
FAU - Govindarajan, Parameswari
AU  - Govindarajan P
FAU - Schlewitz, Gudrun
AU  - Schlewitz G
FAU - Heiss, Christian
AU  - Heiss C
FAU - Janek, Juergen
AU  - Janek J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130717
PL  - England
TA  - J R Soc Interface
JT  - Journal of the Royal Society, Interface
JID - 101217269
RN  - 27YLU75U4W (Phosphorus)
RN  - 9007-34-5 (Collagen)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - *Bone Density
MH  - Calcium/*metabolism
MH  - Collagen/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Lumbar Vertebrae/*metabolism/pathology
MH  - Osteoporosis, Postmenopausal/*metabolism/pathology
MH  - Ovariectomy
MH  - Phosphorus/metabolism
MH  - Rats
MH  - Spectrometry, Mass, Secondary Ion/*methods
MH  - Time Factors
PMC - PMC3730683
OTO - NOTNLM
OT  - bone imaging
OT  - calcium distribution in bone
OT  - chemical imaging
OT  - collagen
OT  - mass spectrometry
EDAT- 2013/07/19 06:00
MHDA- 2014/02/05 06:00
PMCR- 2014/09/06
CRDT- 2013/07/19 06:00
PHST- 2013/07/19 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2014/02/05 06:00 [medline]
PHST- 2014/09/06 00:00 [pmc-release]
AID - rsif.2013.0332 [pii]
AID - rsif20130332 [pii]
AID - 10.1098/rsif.2013.0332 [doi]
PST - epublish
SO  - J R Soc Interface. 2013 Jul 17;10(86):20130332. doi: 10.1098/rsif.2013.0332. 
      Print 2013 Sep 6.