PMID- 23864561 OWN - NLM STAT- MEDLINE DCOM- 20131022 LR - 20130718 IS - 1758-2229 (Electronic) IS - 1758-2229 (Linking) VI - 5 IP - 4 DP - 2013 Aug TI - Association of rhizobia with a marine polychaete. PG - 492-8 LID - 10.1111/1758-2229.12043 [doi] AB - We report the presence of Mesorhizobium, a genus best known for its nitrogen-fixing symbiosis with terrestrial legumes, associated with the marine polychaete Meganerilla bactericola (Annelida: Nerillidae). Abundant epibionts were previously described as coating the exterior of M. bactericola, which is found within the anoxic sulfide-oxidizing microbial mats of the Santa Barbara Basin, California, USA. 16S rRNA investigation of the bacterial community associated with this polychaete discovered the presence of bacteria belonging to Mesorhizobium. We identified these bacteria using phylogenetic analyses of 16S rRNA and three additional functional genes, nifH, atpD and recA, and group-specific fluorescence in situ hybridization (FISH). CI - (c) 2013 John Wiley & Sons Ltd and Society for Applied Microbiology. FAU - Summers, Mindi M AU - Summers MM AD - Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA. msummers@ucsd.edu FAU - Katz, Sigrid AU - Katz S FAU - Allen, Eric E AU - Allen EE FAU - Rouse, Greg W AU - Rouse GW LA - eng SI - GENBANK/KC521436 SI - GENBANK/KC521437 SI - GENBANK/KC521438 SI - GENBANK/KC521439 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20130318 PL - United States TA - Environ Microbiol Rep JT - Environmental microbiology reports JID - 101499207 RN - 0 (DNA, Bacterial) RN - 0 (DNA, Ribosomal) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Transcription Factors) RN - EC 1.- (Oxidoreductases) RN - EC 1.18.6.1 (nitrogenase reductase) RN - EC 2.7.7.- (Rec A Recombinases) SB - IM MH - Animals MH - Aquatic Organisms/*microbiology MH - California MH - Cluster Analysis MH - DNA, Bacterial/chemistry/genetics MH - DNA, Ribosomal/chemistry/genetics MH - In Situ Hybridization, Fluorescence MH - Mesorhizobium/*classification/genetics/*isolation & purification MH - Molecular Sequence Data MH - Oxidoreductases/genetics MH - Phylogeny MH - Polychaeta/*microbiology MH - RNA, Ribosomal, 16S/genetics MH - Rec A Recombinases/genetics MH - Sequence Analysis, DNA MH - Transcription Factors/genetics EDAT- 2013/07/19 06:00 MHDA- 2013/10/23 06:00 CRDT- 2013/07/19 06:00 PHST- 2012/11/21 00:00 [received] PHST- 2013/01/30 00:00 [revised] PHST- 2013/02/12 00:00 [accepted] PHST- 2013/07/19 06:00 [entrez] PHST- 2013/07/19 06:00 [pubmed] PHST- 2013/10/23 06:00 [medline] AID - 10.1111/1758-2229.12043 [doi] PST - ppublish SO - Environ Microbiol Rep. 2013 Aug;5(4):492-8. doi: 10.1111/1758-2229.12043. Epub 2013 Mar 18.