PMID- 23866702
OWN - NLM
STAT- MEDLINE
DCOM- 20140911
LR  - 20130722
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 49
IP  - 3
DP  - 2013 Mar
TI  - [Screening of key genes and inflammatory signalling pathway involved in the 
      pathogenesis of HLA-B27-associated acute anterior uveitis by gene expression 
      microarray].
PG  - 217-23
AB  - OBJECTIVE: To investigate the genes and signalling pathways located upstream of 
      the inflammatory processes in human leukocyte antigen (HLA)-B27-associated acute 
      anterior uveitis by gene expression microarray. METHODS: Experimental study. 
      HLA-B27-positive and-negative monocytes isolated from human peripheral blood were 
      stimulated with Vibrio cholera lipopolysaccharide (LPS). Gene expression 
      microarrays were used to identify the differentially expressed genes. 
      Differentially expressed (DE) genes were testified by real-time PCR and analyzed 
      by a series of bioinformatics-based techniques such as Gene Ontology, Kyoto 
      Encyclopedia of Genes and Genomes. RESULTS: Gene expression microarray analysis 
      revealed marked differences between HLA-B27-positive acute anterior uveitis (AAU) 
      and HLA-B27-negative healthy control peripheral monocytes in the genes that were 
      upregulated in response to LPS stimulation with 1105 genes and 25 genes 
      respectively. Gene Ontology enrichment and pathway analysis indicated that genes 
      participating in protein transport and folding were essential to the inflammatory 
      process. The LPS receptor-Toll-like receptor (TLR)4 induced TLR signalling 
      pathway and pathway related to Vibrio cholerae infection were located upstream of 
      the network and contribute to the overall response. Among the DE genes, PIK3CA, 
      PIK3CB, AKT3, and MAPK1 might play critical roles in inflammation. CONCLUSIONS: 
      Equivalent LPS stimulation induces a different response in HLA-B27-positive 
      peripheral monocytes compared to normal control, suggesting that the TLR pathway 
      is involved in the pathogenesis of HLA-B27-associated AAU.
FAU - Hu, Xiao-feng
AU  - Hu XF
AD  - Eye Department, Capital Medical University, Beijing, China.
FAU - Lu, Hong
AU  - Lu H
FAU - Wang, Jing
AU  - Wang J
FAU - Zhang, Xiao-sheng
AU  - Zhang XS
FAU - Zhang, Xiao-long
AU  - Zhang XL
FAU - Liu, Xu-hui
AU  - Liu XH
FAU - Xu, Zhuo-zai
AU  - Xu ZZ
FAU - Hu, Jun-min
AU  - Hu JM
FAU - Lu, Qing-jun
AU  - Lu QJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 0 (HLA-B27 Antigen)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Female
MH  - Gene Expression Profiling
MH  - HLA-B27 Antigen/*immunology
MH  - Humans
MH  - Inflammation
MH  - Male
MH  - Monocytes/*metabolism
MH  - Signal Transduction/genetics
MH  - Uveitis, Anterior/*blood/genetics/*immunology
EDAT- 2013/07/23 06:00
MHDA- 2014/09/12 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/09/12 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2013 Mar;49(3):217-23.