PMID- 23866781
OWN - NLM
STAT- MEDLINE
DCOM- 20131105
LR  - 20181202
IS  - 1531-5053 (Electronic)
IS  - 0278-2391 (Linking)
VI  - 71
IP  - 9
DP  - 2013 Sep
TI  - A preliminary study on the application of bone marrow stromal cell sheet on the 
      formation of functional tissue-engineered bone in dogs.
PG  - 1531.e1-1531.e10
LID - S0278-2391(13)00431-X [pii]
LID - 10.1016/j.joms.2013.04.029 [doi]
AB  - PURPOSE: The aim of this study was to construct functional tissue-engineered bone 
      in dogs using cell sheet engineering, a new technique to gain and transfer seed 
      cells. MATERIALS AND METHODS: Demineralized bone matrixes, prepared from 
      homologous bone, were coated with recombinant human bone morphogenetic protein-2. 
      Bone marrow stromal cells (BMSCs) were isolated and subcultured. 
      Osteogenic-induced BMSCs were incubated in a temperature-responsive culture dish 
      to form the BMSC sheet. The complex of demineralized bone matrix, recombinant 
      human bone morphogenetic protein-2, and BMSCs wrapped with BMSC sheets was 
      implanted around the blood vessels of the latissimus dorsi muscle in the 
      experimental side, and the same complex without BMSC sheets was implanted around 
      the blood vessels of the latissimus dorsi muscle on the other side as a control. 
      At 4, 8, 12, and 16 weeks after implantation, the implants were removed for 
      radiographic evaluation, descriptive histologic observation, and histologic 
      quantitative analysis. RESULTS: Radiographic analysis showed that the optical 
      density of the tissue-engineered bone on the 2 sides increased with time. 
      However, the optical density of the experimental side was significantly greater 
      than that of the control side at the same points. Sixteen weeks after 
      implantation, mature lamellar bone was formed in the experimental side, with red 
      bone marrow in the bone marrow cavity. In contrast, the control side exhibited 
      significantly less lamellar bone. Histologic quantitative analysis showed that 
      the experimental side exhibited significantly more bone per area compared with 
      the control side. CONCLUSION: BMSC sheet engineering may be useful to construct 
      functional tissue-engineered bone.
CI  - Copyright (c) 2013 American Association of Oral and Maxillofacial Surgeons. 
      Published by Elsevier Inc. All rights reserved.
FAU - Du, Chun-hua
AU  - Du CH
AD  - Central Laboratory, Affiliated Hospital of the Medical College, Qingdao 
      University, Qingdao, China.
FAU - Li, Ning-yi
AU  - Li NY
FAU - Gao, Ning
AU  - Gao N
FAU - Yao, Chao
AU  - Yao C
FAU - Wang, Shuang-yi
AU  - Wang SY
FAU - Bu, Ling-xue
AU  - Bu LX
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130715
PL  - United States
TA  - J Oral Maxillofac Surg
JT  - Journal of oral and maxillofacial surgery : official journal of the American 
      Association of Oral and Maxillofacial Surgeons
JID - 8206428
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Culture Media)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (recombinant human bone morphogenetic protein-2)
SB  - IM
MH  - Animals
MH  - Bone Demineralization Technique
MH  - Bone Marrow Cells/*physiology
MH  - Bone Matrix
MH  - Bone Morphogenetic Protein 2/chemistry
MH  - Bone and Bones/diagnostic imaging/pathology/*physiology
MH  - Capillaries/pathology
MH  - Cell Culture Techniques
MH  - Cell Separation
MH  - Culture Media
MH  - Dogs
MH  - Fascia/blood supply
MH  - Fasciotomy
MH  - Female
MH  - Haversian System/pathology
MH  - Humans
MH  - Humidity
MH  - Image Processing, Computer-Assisted/methods
MH  - Male
MH  - Mesenchymal Stem Cells/*physiology
MH  - Muscle, Skeletal/blood supply/surgery
MH  - Osteoblasts/physiology
MH  - Osteogenesis/*physiology
MH  - Radiography
MH  - Random Allocation
MH  - Recombinant Proteins/chemistry
MH  - Temperature
MH  - Tissue Engineering/instrumentation/*methods
MH  - Tissue Scaffolds/chemistry
MH  - Transforming Growth Factor beta/chemistry
EDAT- 2013/07/23 06:00
MHDA- 2013/11/06 06:00
CRDT- 2013/07/23 06:00
PHST- 2012/10/03 00:00 [received]
PHST- 2013/04/22 00:00 [revised]
PHST- 2013/04/25 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2013/11/06 06:00 [medline]
AID - S0278-2391(13)00431-X [pii]
AID - 10.1016/j.joms.2013.04.029 [doi]
PST - ppublish
SO  - J Oral Maxillofac Surg. 2013 Sep;71(9):1531.e1-1531.e10. doi: 
      10.1016/j.joms.2013.04.029. Epub 2013 Jul 15.