PMID- 23867162
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20131220
IS  - 1661-5433 (Electronic)
IS  - 1661-5425 (Linking)
VI  - 7
IP  - 6
DP  - 2013
TI  - Thermal and endocrine regulation of gonadal apoptosis during sex differentiation 
      in pejerrey Odontesthes bonariensis.
PG  - 316-24
LID - 10.1159/000353506 [doi]
AB  - The fate of the differentiating gonads in pejerrey Odontesthes bonariensis is 
      determined by the environmental water temperature experienced by the larvae 
      during the critical period of sex determination. We previously reported a link 
      between apoptosis, temperature and sex differentiation in this species. To 
      clarify this link, we subjected larvae to thermal and endocrine treatments 
      between hatching and the onset of histological sex differentiation of the gonads 
      and assessed the patterns of gonadal development and apoptosis by light 
      microscopic histology and terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) analysis, respectively. Apoptotic labeling was 
      widespread among somatic cells of the anterior region of the right gonads of fish 
      reared at the male-producing temperature (MPT) and part of the fish at the mixed 
      sex-producing temperature prior to sex differentiation. In contrast, 
      TUNEL-positive cells were rarely observed in gonads at the female-producing 
      temperature (FPT). Administration of exogenous estrogen completely prevented 
      MPT-induced masculinization, induced feminization and reduced gonadal apoptosis, 
      whereas an aromatase inhibitor (fadrozole) induced TUNEL signals in the gonads of 
      FPT-reared larvae. These results provide strong evidence that apoptosis in 
      somatic cells in the right lobe of the gonads might play a key role in testicular 
      differentiation in pejerrey and that estrogens are involved in the regulation of 
      this process.
CI  - (c) 2013 S. Karger AG, Basel.
FAU - Yamamoto, Y
AU  - Yamamoto Y
AD  - Department of Marine Biosciences, Graduate School of Marine Science and 
      Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
FAU - Hattori, R S
AU  - Hattori RS
FAU - Kitahara, A
AU  - Kitahara A
FAU - Kimura, H
AU  - Kimura H
FAU - Yamashita, M
AU  - Yamashita M
FAU - Strussmann, C A
AU  - Strussmann CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130718
PL  - Switzerland
TA  - Sex Dev
JT  - Sexual development : genetics, molecular biology, evolution, endocrinology, 
      embryology, and pathology of sex determination and differentiation
JID - 101316472
RN  - 0 (Aromatase Inhibitors)
RN  - 0 (Estrogens)
RN  - 0 (Gonadal Steroid Hormones)
RN  - H3988M64PU (Fadrozole)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Aromatase Inhibitors/pharmacology
MH  - Estrogens/pharmacology/physiology
MH  - Fadrozole/pharmacology
MH  - Female
MH  - Fishes/*growth & development
MH  - Gonadal Steroid Hormones/*physiology
MH  - Gonads/*cytology
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Sex Differentiation/drug effects/*physiology
MH  - *Temperature
MH  - Testis/growth & development
EDAT- 2013/07/23 06:00
MHDA- 2014/09/03 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/05/05 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - 000353506 [pii]
AID - 10.1159/000353506 [doi]
PST - ppublish
SO  - Sex Dev. 2013;7(6):316-24. doi: 10.1159/000353506. Epub 2013 Jul 18.