PMID- 23868865
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20220311
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 10
IP  - 6
DP  - 2016 Jun
TI  - Characterization of chondrocyte sheets prepared using a co-culture method with 
      temperature-responsive culture inserts.
PG  - 486-95
LID - 10.1002/term.1764 [doi]
AB  - Conventional culture methods using temperature-responsive culture dishes require 
      4-5 weeks to prepare layered chondrocyte sheets that can be used in articular 
      cartilage repair and regeneration. This study investigated whether the use of 
      synovial tissue obtained from the same joint as the chondrocyte nutritive supply 
      source could more quickly facilitate the preparation of chondrocyte sheets. After 
      culturing derived synoviocytes and chondrocytes together (i.e. combined culture 
      or co-culture) on temperature-responsive inserts, chondrocyte growth was assessed 
      and a molecular analysis of the chondrocyte sheets was performed. Transplantable 
      tissue could be obtained more quickly using this method (average 10.5 days). 
      Real-time polymerase chain reaction and immunostaining of the three-layer 
      chondrocyte sheets confirmed the significant expression of genes critical to 
      cartilage maintenance, including type II collagen (COL2), aggrecan-1 and tissue 
      metallopeptidase inhibitor 1. However, the expression of COL1, matrix 
      metalloproteinase 3 (MMP3), MMP13 and A-disintegrin and metalloproteinase with 
      thrombospondin motifs 5 was suppressed. The adhesive factor fibronectin-1 (FN1) 
      was observed in all sheet layers, whereas in sheets generated using conventional 
      preparation methods positive FN1 immunostaining was observed only on the surface 
      of the sheets. The results indicate that synoviocyte co-cultures provide an 
      optimal environment for the preparation of chondrocyte sheets for tissue 
      transplantation and are particularly beneficial for shortening the required 
      culture period. Copyright (c) 2013 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Kokubo, Mami
AU  - Kokubo M
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Isehara, Kanagawa, Japan.
FAU - Sato, Masato
AU  - Sato M
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Isehara, Kanagawa, Japan.
FAU - Yamato, Masayuki
AU  - Yamato M
AD  - Institute of Advanced Biomedical Engineering and Science, Tokyo, Women's Medical 
      University, Shinjuku-ku, Tokyo, Japan.
FAU - Mitani, Genya
AU  - Mitani G
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Isehara, Kanagawa, Japan.
FAU - Kutsuna, Toshiharu
AU  - Kutsuna T
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Isehara, Kanagawa, Japan.
FAU - Ebihara, Goro
AU  - Ebihara G
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Isehara, Kanagawa, Japan.
FAU - Okano, Teruo
AU  - Okano T
AD  - Institute of Advanced Biomedical Engineering and Science, Tokyo, Women's Medical 
      University, Shinjuku-ku, Tokyo, Japan.
FAU - Mochida, Joji
AU  - Mochida J
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Isehara, Kanagawa, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130719
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Adult
MH  - Antigens, Differentiation/*biosynthesis
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/*metabolism
MH  - Coculture Techniques
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Male
OTO - NOTNLM
OT  - chondrocyte
OT  - co-culture
OT  - regeneration
OT  - synovial cells
OT  - temperature-responsive culture dishes
OT  - three-layer chondrocyte sheets
EDAT- 2013/07/23 06:00
MHDA- 2017/11/29 06:00
CRDT- 2013/07/23 06:00
PHST- 2012/09/11 00:00 [received]
PHST- 2013/02/02 00:00 [revised]
PHST- 2013/04/13 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - 10.1002/term.1764 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2016 Jun;10(6):486-95. doi: 10.1002/term.1764. Epub 2013 
      Jul 19.