PMID- 23869602
OWN - NLM
STAT- MEDLINE
DCOM- 20140429
LR  - 20211021
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 54
IP  - 3
DP  - 2014 Mar
TI  - In vitro measures of membrane changes reveal differences between red blood cells 
      stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired 
      study.
PG  - 560-8
LID - 10.1111/trf.12344 [doi]
AB  - BACKGROUND: Saline-adenine-glucose-mannitol (SAGM) and a variant solution, AS-1, 
      have been used for more than 30 years to preserve red blood cells (RBCs). 
      Reputedly these RBC components have similar quality, although no paired study has 
      been reported. To determine whether differences exist, a paired study of SAGM 
      RBCs and AS-1 RBCs was conducted to identify membrane changes, including 
      microparticle (MP) quantitation and in vitro RBC-endothelial cell (EC) 
      interaction. STUDY DESIGN AND METHODS: Two whole blood packs were pooled and 
      split and RBCs were prepared (n=6 pairs). One pack was suspended in SAGM and one 
      in AS-1. Samples were collected during 42 days of refrigerated storage. RBC shape 
      and size and glycophorin A (GPA)(+) and phosphatidylserine (PS)(+) MPs were 
      measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro 
      flow perfusion assay. Routine variables (pH, hemolysis) were also measured. 
      RESULTS: Compared to SAGM RBCs, AS-1 RBCs had lower hemolysis (p<0.04), lower 
      GPA(+) MPs (p<0.03), and lower PS(+) MPs (p<0.03) from Day 14 onward. AS-1 RBCs 
      had higher (p<0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM 
      RBCs were more adherent to ECs on Day 28 of storage compared to AS-1 RBCs 
      (p=0.04), but reversed on Day 42 (p=0.02). CONCLUSION: SAGM RBCs lose more 
      membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of 
      storage, while AS-1 RBCs were more adherent on Day 42. The effect of these 
      differences on the function and survival of SAGM RBCs and AS-1 RBCs after 
      transfusion remains to be determined.
CI  - (c) 2013 Australian Red Cross. Transfusion (c) 2013 American Association of Blood 
      Banks.
FAU - Sparrow, Rosemary L
AU  - Sparrow RL
AD  - Research and Development, Australian Red Cross Blood Service, Melbourne, 
      Victoria, Australia; Blood Systems Research Institute, San Francisco, California.
FAU - Sran, Amrita
AU  - Sran A
FAU - Healey, Geraldine
AU  - Healey G
FAU - Veale, Margaret F
AU  - Veale MF
FAU - Norris, Philip J
AU  - Norris PJ
LA  - eng
GR  - R01 HL095470/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130722
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 3OWL53L36A (Mannitol)
RN  - IY9XDZ35W2 (Glucose)
RN  - JAC85A2161 (Adenine)
SB  - IM
CIN - Transfusion. 2014 Mar;54(3):501-2. PMID: 24617585
MH  - Adenine/*pharmacology
MH  - Adult
MH  - Blood Preservation/*methods
MH  - Erythrocytes/*drug effects
MH  - Flow Cytometry
MH  - Glucose/*pharmacology
MH  - Humans
MH  - Mannitol/*pharmacology
MH  - Middle Aged
MH  - Young Adult
PMC - PMC4173075
MID - NIHMS613278
EDAT- 2013/07/23 06:00
MHDA- 2014/04/30 06:00
PMCR- 2015/03/01
CRDT- 2013/07/23 06:00
PHST- 2013/03/15 00:00 [received]
PHST- 2013/05/17 00:00 [revised]
PHST- 2013/05/29 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/04/30 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - 10.1111/trf.12344 [doi]
PST - ppublish
SO  - Transfusion. 2014 Mar;54(3):560-8. doi: 10.1111/trf.12344. Epub 2013 Jul 22.