PMID- 23871214
OWN - NLM
STAT- MEDLINE
DCOM- 20140403
LR  - 20191210
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 155
IP  - 1-2
DP  - 2013 Sep 1
TI  - Functional characterization of porcine LSm14A in IFN-beta induction.
PG  - 110-6
LID - S0165-2427(13)00201-8 [pii]
LID - 10.1016/j.vetimm.2013.06.019 [doi]
AB  - Human LSm14A has recently been found as a processing body-associated sensor of 
      intracellular viral nucleic acids and triggers signaling for type I IFN 
      expression. Here porcine LSm14A (pLSm14A) was cloned from the PK-15 cells. The 
      pLSm14A ORF is 1392 bp in length, encoding 463 amino acids. The putative pLSm14A 
      contains a Sm-like domain and two arginine-glycine-glycine (RGG) boxes. The 
      pLSm14A has high identity at the amino acid level to those of bovine, human and 
      mouse (93.5-97.4%) and is transcribed in different tissues of pigs. In HEK293 or 
      Marc-145 cells, pLSm14A was localized in the cytosol as P-body-like dots. 
      Expression of pLSm14A in HEK293 or Marc-145 cells enhanced activities of IFN-beta 
      and NF-kappaB promoters, induced IFN-beta transcription, and potentiated 
      poly(I:C)-induced IFN-beta promoter activation, indicating that pLSm14A is a 
      potential signal molecule in the IFN-beta pathway of pigs. We also found that 
      pLSm14A-induced IFN-beta promoter activity was down-regulated by porcine 
      reproductive and respiratory syndrome virus infection in Marc-145 cells. Since 
      pLSm14A is constitutively expressed in virtually all tissues, more research is 
      needed to explore its role in initial phase of viral infections of pigs and its 
      relationship with RIG-I in sensing PAMPs for type I IFN induction.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Wen, Guilan
AU  - Wen G
AD  - Zhejiang University Institute of Preventive Veterinary Medicine, Zhejiang 
      Province Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China.
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Zhang, Xian
AU  - Zhang X
FAU - Hu, Hongxia
AU  - Hu H
FAU - Zhang, Hansong
AU  - Zhang H
FAU - Cheng, Changyong
AU  - Cheng C
FAU - Wang, Xiaodu
AU  - Wang X
FAU - Li, Xiaoliang
AU  - Li X
FAU - Fang, Weihuan
AU  - Fang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130703
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - HEK293 Cells
MH  - Humans
MH  - Immunity, Innate
MH  - Interferon-beta/*biosynthesis/*genetics
MH  - Mice
MH  - NF-kappa B/genetics
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/immunology/pathogenicity
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins/*genetics/*immunology/metabolism
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Signal Transduction
MH  - Sus scrofa/*genetics/*immunology/metabolism
MH  - Tissue Distribution
OTO - NOTNLM
OT  - Porcine LSm14A
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Type I interferon
EDAT- 2013/07/23 06:00
MHDA- 2014/04/04 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/06/08 00:00 [revised]
PHST- 2013/06/27 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/04/04 06:00 [medline]
AID - S0165-2427(13)00201-8 [pii]
AID - 10.1016/j.vetimm.2013.06.019 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2013 Sep 1;155(1-2):110-6. doi: 
      10.1016/j.vetimm.2013.06.019. Epub 2013 Jul 3.