PMID- 23871220
OWN - NLM
STAT- MEDLINE
DCOM- 20131106
LR  - 20191210
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 444
IP  - 1-2
DP  - 2013 Sep
TI  - Development and application of a blocking enzyme-linked immunosorbent assay 
      (ELISA) to differentiate antibodies against live and inactivated porcine 
      reproductive and respiratory syndrome virus.
PG  - 310-6
LID - S0042-6822(13)00397-8 [pii]
LID - 10.1016/j.virol.2013.06.027 [doi]
AB  - The aim of this study was to establish a method that could differentiate 
      antibodies against live and inactivated vaccines of porcine reproductive and 
      respiratory syndrome virus (PRRSV). A blocking ELISA (b-ELISA) was established 
      using the PRRSV non-structural protein, Nsp9, as the antigen and a monoclonal 
      antibody, 2D6, against the Nsp9 protein as the capture antibody. The test was 
      validated by using 415 clinical sera in the b-ELISA compared to a commercial kit 
      based on the indirect ELISA using the nucleocapsid (N) protein as antigen. 
      Significant differences were observed for the data obtained by the two detection 
      methods. This may be due to the commercial kit detecting antibodies elicited by 
      live and inactivated virus, whereas the b-ELISA only detects antibodies produced 
      by any active viral replication, such as natural infection or live vaccination. 
      Therefore, the b-ELISA in this study is able to distinguish between antibodies 
      against live and inactivated viruses in pigs.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Cong, Yanlong
AU  - Cong Y
AD  - College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
FAU - Huang, Zhiqiang
AU  - Huang Z
FAU - Sun, Yixue
AU  - Sun Y
FAU - Ran, Wei
AU  - Ran W
FAU - Zhu, Lisai
AU  - Zhu L
FAU - Yang, Guilian
AU  - Yang G
FAU - Ding, Xuemei
AU  - Ding X
FAU - Yang, Zhanqing
AU  - Yang Z
FAU - Huang, Xiao
AU  - Huang X
FAU - Wang, Chunfeng
AU  - Wang C
FAU - Ding, Zhuang
AU  - Ding Z
LA  - eng
SI  - GENBANK/KF049009
PT  - Journal Article
PT  - Validation Study
DEP - 20130718
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/*blood/immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/classification/*immunology
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/immunology
MH  - Vaccines, Inactivated/administration & dosage/immunology
MH  - Viral Nonstructural Proteins/immunology
MH  - Viral Vaccines/administration & dosage/*immunology
OTO - NOTNLM
OT  - Blocking ELISA
OT  - Monoclonal antibodies
OT  - Nsp9
OT  - PRRSV
EDAT- 2013/07/23 06:00
MHDA- 2013/11/07 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/04/11 00:00 [received]
PHST- 2013/05/04 00:00 [revised]
PHST- 2013/06/25 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2013/11/07 06:00 [medline]
AID - S0042-6822(13)00397-8 [pii]
AID - 10.1016/j.virol.2013.06.027 [doi]
PST - ppublish
SO  - Virology. 2013 Sep;444(1-2):310-6. doi: 10.1016/j.virol.2013.06.027. Epub 2013 
      Jul 18.