PMID- 23871284 OWN - NLM STAT- MEDLINE DCOM- 20140213 LR - 20130802 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1304 DP - 2013 Aug 23 TI - Study on steric transition in asymmetrical flow field-flow fractionation and application to characterization of high-energy material. PG - 211-9 LID - S0021-9673(13)00962-X [pii] LID - 10.1016/j.chroma.2013.06.051 [doi] AB - In field-flow fractionation (FFF), there is the 'steric transition' phenomenon where the sample elution mode changes from the normal to steric/hyperlayer mode. Accurate analysis by FFF requires understanding of the steric transition phenomenon, particularly when the sample has a broad size distribution, for which the effect by combination of different modes may become complicated to interpret. In this study, the steric transition phenomenon in asymmetrical flow FFF (AF4) was studied using polystyrene (PS) latex beads. The retention ratio (R) gradually decreases as the particle size increases (normal mode) and reaches a minimum (Ri) at diameter around 0.5mum, after which R increases with increasing diameter (steric/hyperlayer mode). It was found that the size-based selectivity (Sd) tends to increase as the channel thickness (w) increases. The retention behavior of cyclo-1,3,5-trimethylene-2,4,6-trinitramine (commonly called 'research department explosive' (RDX)) particles in AF4 was investigated by varying experimental parameters including w and flow rates. AF4 showed a good reproducibility in size determination of RDX particles with the relative standard deviation of 4.1%. The reliability of separation obtained by AF4 was evaluated by transmission electron microscopy (TEM). CI - Copyright (c) 2013 Elsevier B.V. All rights reserved. FAU - Dou, Haiyang AU - Dou H AD - Department of Chemistry, Hannam University, Daejeon 305-811, South Korea. FAU - Lee, Yong-Ju AU - Lee YJ FAU - Jung, Euo Chang AU - Jung EC FAU - Lee, Byung-Chul AU - Lee BC FAU - Lee, Seungho AU - Lee S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130628 PL - Netherlands TA - J Chromatogr A JT - Journal of chromatography. A JID - 9318488 RN - 0 (Explosive Agents) RN - 0 (Polystyrenes) RN - 0 (Triazines) RN - W91SSV5831 (cyclonite) SB - IM MH - Algorithms MH - Explosive Agents/*isolation & purification MH - Fractionation, Field Flow/*methods MH - Microspheres MH - Particle Size MH - Polystyrenes/chemistry MH - Reproducibility of Results MH - Triazines/*isolation & purification OTO - NOTNLM OT - Asymmetrical flow field-flow fractionation (AF4) OT - Normal mode OT - RDX particles OT - Steric transition OT - Steric/hyperlayer mode EDAT- 2013/07/23 06:00 MHDA- 2014/02/14 06:00 CRDT- 2013/07/23 06:00 PHST- 2013/05/12 00:00 [received] PHST- 2013/06/17 00:00 [revised] PHST- 2013/06/19 00:00 [accepted] PHST- 2013/07/23 06:00 [entrez] PHST- 2013/07/23 06:00 [pubmed] PHST- 2014/02/14 06:00 [medline] AID - S0021-9673(13)00962-X [pii] AID - 10.1016/j.chroma.2013.06.051 [doi] PST - ppublish SO - J Chromatogr A. 2013 Aug 23;1304:211-9. doi: 10.1016/j.chroma.2013.06.051. Epub 2013 Jun 28.